Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis viruses of humans and animals. These agents have been reported from several countries of the world and represent an important cause of economic loss. The Amazon area has a high degree of diversity of animals and plants, is located in the Northern Region of Brazil and accounts for a large part of the Brazilian territory. In this study, stool samples were collected from pigs during the phase of nursing (less than 28 days of age) and post-weaning (29 to 56 days of age) from January 2008 to February 2009. A total of 169 specimens (108 nursing and 61 post-weaning pigs) were tested by reverse transcription polymerase chain reaction (RT-PCR) using the primers p289/p290 for the detection of caliciviruses (CVs), i.e., SaVs and noroviruses (NoVs). Positive sequences were analyzed using BioEdit software (v. 7.1.3.0) and compared with other sequences registered in the GenBank database. A positive frequency of 12.4 % (21/169) was observed, and all of the viruses found were identified as SaVs, with 15 belonging to genogroup GIII (71.4 %), three to GVII-1 (14.3 %) and three to GVIII-2 (14.3 %). No NoVs were detected. The frequency of SaV infections was significantly higher in nursing pigs (17.6 %-19/108) than in post-weaning pigs (3.3 %-2/61). Considering the consistency of the samples, 14.7 % of the samples were classified as diarrheic, but statistical analysis demonstrated that there was no significant difference compared to normal specimens (p = 0.5795). For the first time, we have demonstrated the circulation of SaVs in pigs from the Amazon.

Rotaviruses are an important agent of diarrhea in piglets, primarily during nursing and post-weaning with severity. The aim of this study was to identify rotavirus G and P genotypes obtained from piglets reared at five piggeries in Belém, Pará State, Northern Brazil. Fecal specimens were collected from nursing and weaned piglets. A total of 172 samples were tested, of which 17 (9.9%) were positive for group A rotaviruses all of them from piglets kept at nursing and then were sequenced for the of VP7 and VP4 genotyping. The consistency of positive fecal samples were 53% (9/17) diarrheic, 23.5% (4/17) pasty and 23.5% (4/17) normal. The most common G genotype was G3 representing 53% (9/17), followed by G5 genotype (17%, 3/17). The P genotype recorded was P[23] corresponding to 23.5% (4/17). This study showed that rotaviruses circulated in swine herds in the metropolitan region of Belém, in Northern region. G3P[23] combination was recorded for the first time in Brazil.

O objetivo do presente estudo foi determinar a prevalência de anticorpos em um rebanho de búfalos de rio da Ilha de Marajó para os vírus: Vírus da diarreia viral bovina, Herpesvírus bovino tipo 1 e Vírus da leucose enzoótica bovina. A técnica de soroneutralização foi  utilizada para detecção de anticorpos referentes aos Vírus da diarreia viral bovina e Herpesvírus bovino tipo 1 e o teste de imunodifusão em gel de ágar para detecção de anticorpos para o Vírus da leucose enzoótica bovina. A prevalência de anticorpos para o Herpesvírus bovino tipo 1 em bubalinos está muito próxima da prevalência observada em bovinos. O Vírus da diarreia viral bovina apresentou prevalência de anticorpos muito abaixo da média quando comparada com prevalência de anticorpos em bovinos e bubalinos, inclusive criados no Brasil. O Vírus da leucose enzoótica bovina mostrou prevalência de anticorpos muito baixa, porém, próxima dos parâmetros observados em búfalos no Brasil e no mundo, que divergem da prevalência observada em bovinos, mostrando provavelmente uma resistência natural dessa espécie ao vírus, fator esse que precisa ser mais bem elucidado.