ObjectiveTo determine the activities of oxidative stress markers and lipid profiles of patients with polycystic ovary syndrome (PCOS) in Nnamdi Azikiwe University Teaching Hospital Nnewi, Nigeria.MethodsThis was a nested case-control study consisting of 50 PCOS patients and 50 healthy women of the same age range without any evidence of PCOS. The study measured the levels of malondialdehyde (MDA), activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (TAC); concentrations of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), as well as high-density lipoprotein cholesterol (HDL-C) using standard spectrophotometric methods. Anthropometric indices were also assessed. P-values of <0.05 were taken to be statistically significant.ResultsThere were significantly higher levels of MDA (P=0.002), lower activity of SOD (P<0.001), and lower TAC (P=0.001) in PCOS patients when compared with the controls. There were higher concentrations of TC (P=0.017) and LDL-C P=0.012) in PCOS patients than in controls. Significant differences were not observed between the 2 groups in terms of the activity of GSH-Px, as well as the concentrations of HDL-C and TG. The body mass index, waist circumference, and waist-hip ratio were all significantly higher in PCOS patients.ConclusionThis study revealed higher levels of MDA, as well as lower activity of SOD and TAC, among PCOS patients. Furthermore, there were higher levels of TC and LDL-C among the PCOS patients. Hence, monitoring these parameters may improve the clinical management of PCOS.
Objective: To determine the possible association between cadmium (Cd) exposure and alterations in plasma lipid and lipoprotein levels and total antioxidant status (TAS) in rats. Materials and Method: Twenty male albino rats assigned to 2 groups of 10 rats each (test and control) were used for the study. Each test animal model was given 1.0 mg CdCl2/kg body weight, administered intraperitoneally for 4 weeks. Blood samples obtained from both groups at the beginning (baseline) and after 4 weeks of Cd exposure were analysed for triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), TAS and Cd. Kidney and liver sections from the rats were examined. Results: The plasma TG, TC and LDLC levels were significantly higher in exposed rats than controls (p < 0.0001, p < 0.0001 and p < 0.001, respectively). Plasma HDLC was reduced in the exposed rats compared to controls (0.41 ± 0.22 and 0.68 ± 0.14 mmol/l, respectively). Conclusion: The observed dyslipidaemia and decrease in TAS could be due to increased free radical production causing oxidative stress. Damage to liver and kidney sections in the exposed group suggests that Cd toxicity results in detrimental histological changes and may be implicated in the aetiology of cerebrovascular diseases.
Objective: To evaluate the serum C-reactive protein (CRP) and lipid profile in women with pre-eclampsia. Materials and Methods: Thirty-five women with and 35 women without pre-eclampsia, who were in the 3rd trimester of pregnancy, were enrolled in this study. Weight in kilogrammes and height in metres were measured to calculate the mean body mass index (BMI) for each group. The diastolic and systolic blood pressures were measured. Lipid profile tests and serum CRP assay were done for all patients. Total cholesterol, triglycerides (TG) and high-density lipoprotein cholesterol (HDL-C) were determined using enzymatic methods, while low-density lipoprotein cholesterol (LDL-C) was calculated using Friedewald's formula. Results: The mean values of the BMI were 29.47 ± 6.90 versus 26.14 ± 2.92, of the diastolic blood pressure 109.14 ± 15.41 versus 72.29 ± 9.42 mm Hg and of the systolic blood pressure 170.57 ± 19.55 versus 120.86 ± 17.72 mm Hg for women with and without pre-eclampsia, respectively, and the differences were statistically significant (p = 0.012, p = 0.001 and p = 0.001, respectively). The biochemical analysis also indicated that the women with pre-eclampsia had a significantly higher mean serum CRP (8.57 ± 2.68 vs. 6.46 ± 2.46 mg/l, p = 0.001), TG (2.84 ± 0.45 vs. 1.87 ± 0.38 mmol/l, p = 0.001) and total cholesterol (5.59 ± 0.92 vs. 4.63 ± 0.78 mmol/l, p = 0.001) level but a lower mean HDL-C (1.10 ± 0.12 vs. 1.26 ± 0.15 mmol/l, p = 0.001) level than the controls. There was no statistical difference in the mean LDL-C values between the 2 groups (1.58 ± 0.8 vs. 1.45 ± 0.78 mmol/l, p > 0.05). Conclusion: Significant changes in CRP as well as TG, total cholesterol and HDL-C were associated with pre-eclampsia in these Southeastern Nigerian women.
Objective: To investigate the effect of coffee consumption on some liver function indices in adult male and female Nigerians. Subjects and Methods: Thirty apparently healthy subjects, consisting of 18 men and 12 women, were made to consume 2 g of coffee daily for a total of 30 days. Activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and plasma concentrations of total and conjugated bilirubin, total protein and albumin were determined using standard methods. Results: Relative to baseline values, coffee consumption raised mean levels of ALT by 4 IU/l (p < 0.001), AST by 2.0 1 U/l (p < 0.001), ALP by 3.0 1 U/l (p < 0.01), total bilirubin by 0.90 mg/dl (p < 0.05) and total protein by 1.1 g/l (p < 0.05). Gender differences were observed. Significantly higher mean ALP concentration was only seen in male subjects, while mean bilirubin concentration was significantly raised in female volunteers alone. On the other hand, the mean total protein and albumin concentrations in individual male and female groups were not significantly altered (p > 0.05 in each case). Conclusion: The result obtained from the study suggests that short-term consumption of coffee might have a significant effect on the integrity of the liver function tests studied.
Background: Lead toxicity is one of the most common occupational hazards that affect several organs of the body, kidney inclusive. Auto repairers are exposed to lead in petrol, radiator, leaded battery, lead soldering wire, and spray paints, thus this study was designed to evaluate lead-induced nephrotoxic effect among automobile repairers. Methods: A total of 80 male subjects within the age range of 20 and 65 years were recruited for this study. 50 subjects were occupationally exposed automobile repairers, of which 15 were electricians, 21 mechanics and 14 panel beaters/spray painters, whereas 30 were non-exposed students and staff from Nnamdi Azikiwe University, Nnewi campus. Blood sample was collected from these individuals and their blood lead levels were determined alongside creatinine, urea, uric acid, sodium, potassium, chloride and bicarbonate. Results: The results showed that the mean levels of blood lead is significantly higher in automobile repairers than in control group (P <0.05). The comparison of blood lead level among different categories of automobile repairers (electricians, mechanics, and panel beaters/spray painters) showed no significant difference (P >0.05). The evaluation of the renal function markers show that, there were significant increases in the mean serum concentration of creatinine, urea, and uric acid in the study group compare to the control subjects (P <0.05). However, there was no significance difference in the mean levels of sodium, potassium, chloride, and bicarbonate in study group when compared to the control group (P >0.05). Conclusion: Findings from this study show that blood lead level is high among automobile repairers above CDC recommended level for adults. This high blood lead level among automobile repairers may be responsible for raised levels of renal markers which may eventually lead to their renal damage.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.