In Southeast Asian countries, Chloroquine and Primaquine combination therapy is most successful in treating uncomplicated Plasmodium vivax infections, for preventing relapses.Later, resistance to Chloroquine has emerged resulting in therapeutic failures, and this encouraged the research to identify causative factors (1). Several molecular methods have been developed to identify the genetic mechanisms involved in resistance to chloroquine and other

Malaria is considered as a neglected tropical disease because of the therapeutic failures and lack of immunoprophylactic methods. Plasmodium species develop resistant genes by selective drug pressure, detection of such resistant parasitic strains by various molecular methods is significant in improving treatment modalities. Pvcrt-o, Pvmdr1 in Plasmodium vivax and Pfcrt-o in Plasmodium falciparum are the genes responsible for antimalarial resistance. In this study