Increased consumer demand for fresh leafy produce has been paralleled by an increase in outbreaks and illness associated with these foods. Presently, data on the microbiological quality and safety of produce harvested in the Lower Mainland of British Columbia is lacking. Therefore, fresh green, red, and romaine lettuce samples (n = 68) were obtained from five regional farmers' markets in late summer of 2012 and subsequently analyzed to determine total numbers of aerobic bacteria, coliforms, and Escherichia coli. Additionally, enrichment procedures were used to detect low concentrations of E. coli. Obtained E. coli isolates were subjected to multiplex PCRs to determine phylogenetic groupings and the presence of virulence genes (eaeA, hlyA, stx1, and stx2). All E. coli were tested for resistance to 15 antibiotics using a disk diffusion assay. Lettuce samples yielded mean aerobic colony counts of 6.3 log CFU/g. Coliforms were detected in 72% of samples, with a median concentration of 1.9 log CFU/g. Of samples, 13% were found to harbor E. coli, with a median level of 0.7 log CFU/g. Antibiogram typing of all E. coli (n = 33) revealed that 97% possessed resistance to one or more antimicrobials, with resistance to amikacin (58%), trimethoprim (48%), and trimethoprim-sulfamethoxazole (45%) being the most common. Phylogroup typing showed that 79% of these isolates belonged to group B1, with the remaining assigned to groups A (9%) or D (12%); no virulence genes were detected. Considering that phylogroup indicators suggestive of fecal contamination (groups A and D E. coli) were recovered in lettuce samples presented at retail, further work is required to explore at what point along the food chain contamination occurs. Also, this study shows the presence of multidrug-resistant E. coli in fresh vegetables. Summed, these data provide important information on the microbiological quality of leafy vegetables grown in British Columbia through the detection and characterization of frequently used indicator organisms.
Steps taken to make legal information available online have resulted in access to justice benefits for many. However, these benefits may not extend to everyone equally. As scholars have cautioned, the adoption of new technologies that purport to improve access to justice may perpetuate the exclusion of vulnerable and marginalized individuals and groups from the justice system. This article applies this insight to legal information made available online by Canadian court websites and CanLII.It does so through a two-part study. First, we used an automated testing tool to determine whether the websites noted above comply with accessibility standards. Second, after having secured research ethics approval, we worked with Access & Diversity at the University of British Columbia to recruit persons with visual impairments; these participants evaluated the same websites and provided feedback. Our results showed that while largely accessible, the tested websites fall short of best practices, presenting challenges to users with visual impairments. We recommend that Canadian courts correct the deficiencies identified by our study, that other online legal resources be tested for accessibility issues, and that future research focus on the extent to which online legal resources are accessible to other vulnerable or marginalized individuals or groups. Implementing these recommendations will ensure that the access to justice benefits of online legal information are extended to everyone.
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