Multidrug-resistant (MDR) strains of Salmonella enterica serotype Newport have been described for many years. However, the recognition of Newport strains with resistance to cephalosporin antibiotics is more recent. Plasmid-mediated CMY-2 AmpC ␤-lactamases have been identified in Salmonella in the United States, and the bla CMY-2 gene has been shown to be present in Salmonella serotype Newport. This organism is currently undergoing epidemic spread in both animals and humans in the United States, and this is to our knowledge the first description of the molecular epidemiology of this Salmonella strain in animals. Forty-two isolates were included in this study. All isolates were characterized by pulsed-field gel electrophoresis, plasmid analysis, and antibiogram. Four pulsed-field profiles with XbaI were observed. Plasmid analyses showed that although the majority of isolates harbored a single plasmid of 140 kb, this plasmid was not identical in all strains. All isolates showed the presence of the bla CMY gene by PCR. Integrons were detected in 16 of the 42 isolates; a fragment of approximately 1,000 bp, amplified with the intI-F and aadAI-R primers, confirmed the presence of the aadAI gene cassette within an integron in these 16 isolates. The potential for coselection of the bla CMY gene, if located on an MDR replicon, may not be dependent on any particular antibiotic but rather may be the result of more general antimicrobial use. If this replicon is mobile, it is to be expected that similar MDR strains of additional Salmonella serotypes will be recognized in due course.

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The objective of the Ground to Space CALibration Experiment (G-SCALE) is to demonstrate the use of convex mirrors as a radiometric and spatial calibration and validation technology for Earth Observation assets, operating at multiple altitudes and spatial scales. Specifically, point sources with NIST-traceable absolute radiance signal are evaluated for simultaneous vicarious calibration of multi- and hyperspectral sensors in the VNIR/SWIR range, aboard Unmanned Aerial Vehicles (UAVs), manned aircraft, and satellite platforms. We introduce the experimental process, field site, instrumentation, and preliminary results of the G-SCALE, providing context for forthcoming papers that will detail the results of intercomparison between sensor technologies and remote sensing applications utilizing the mirror-based calibration approach, which is scalable across a wide range of pixel sizes with appropriate facilities. The experiment was carried out at the Rochester Institute of Technology’s Tait Preserve in Penfield, NY, USA on 23 July 2021. The G-SCALE represents a unique, international collaboration between commercial, academic, and government entities for the purpose of evaluating a novel method to improve vicarious calibration and validation for Earth Observation.

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