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h i g h l i g h t s Designed-bioactive contaminants assessed in 5 southeast US NPSprotected streams. 334 unique pesticides and pharmaceuticals were assessed in water; 24% were detected. 119 sediment pesticides assessed; 5 detected consistently but only in one stream. Common exceedances of effectsscreening threshold raise sub-lethal effects concerns. Importance of up-gradient external sources suggest increased community engagement.

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Experimental testing of ecological theory in the field is often difficult because of the number of factors influencing the test system. Adequate control of relevant variables may require the use of laboratory microcosms for the performance of proper experimental tests. Our efforts to study the effects of species invasion sequence on community structure and ecosystem properties of the developing system, requires that the experimental systems be closed. To meet our demanding experimental requirements, we have designed a closed artificial stream that permits careful control over algal species introductions while minimizing the risks of contamination by unwanted species. In assessing the performance of these streams, we examined the variability among experimental replicates in water chemistry parameters and biomass accumulation. Those water chemistry parameters that were not greatly influenced by biological processes in the streams (Ca, Mg, bicarbonate, and sulfate) had average coefficients of variation less than 10%. Those parameters that were clearly influenced by biological processes (P, N, Fe, and Si) had average coefficients of variation ranging from l3.5%-40.4%. Ash free dry mass had an average coefficient of variation of 23.3%, but chlorophyll a was much more variable at 183%. This pattern of variation indicates that the artificial streams performed well in terms of replicability, but some experimental goals may require carefully conceived designs that adequately partition sources of variation in biologically mediated variables to obtain satisfactory results.