No abstract

SUMMARY The use of medicinal plants as a therapeutic method in the control of diseases has been increasing in animal production. In the case of small ruminants, the endoparasitic disease is a major concern, since they are considered the greater sanitary problem, especially when considering the phenomenon of parasite resistance to the medicines used. Therefore, the development of alternative methods of endoparasitary control in goats and sheep has become a pressing need. The use of plants to control endoparasites can reduce the use of chemical inputs, making more environmentally sustainable livestock, minimizing the occurrence of parasitic resistance to conventional medicines and contributing to the reduction of production costs. In this sense, several studies have been carried out in order to evaluate the in vitro and in vivo activity of plants with anthelmintic potential. However, despite the potentiality found in many studies, the results are sometimes contradictory or do not replicate in vivo the same efficacy obtained in the in vitro assays. Another relevant aspect is the low utilization of the lethality and toxicity tests, which are indispensable so that the knowledge can be spread and applied by the producers in their herds. Thus, this review aims to provide the results from studies carried out in Brazil with extract of plants with potential for control of parasitic disease in goats and sheep, describing the main evaluated plants species, mechanisms of action, preparation forms and tests of efficacy, toxicity, and lethality.

Abstract. This study examined the effects of cottonseed processing form and the inclusion of calcium lignosulfonate in high-concentrate diets for feedlot cull ewes on carcass traits and non-carcass components. Thirty Santa Inês cull ewes with an average body weight of 44.2 ± 5.2 kg and an average age of 50 months were distributed into collective stalls in a completely randomized design. The treatments consisted of diets including whole cottonseed, crushed cottonseed, whole cottonseed treated with lignosulfonate (100 g/kg, as fed), crushed cottonseed treated with lignosulfonate (100 g/kg, as fed), and a control diet without cottonseed. The experimental diets did not influence (P>0.05) average daily weight gain (0.195 kg/day), slaughter weight (51.74 kg), or in vivo biometric and on-carcass measurements. There was no difference (P>0.05) in loin-eye area or subcutaneous fat thickness as evaluated in vivo by ultrasound. There was no diet effect on hot carcass weight and yield (24.8 kg and 47.8 %), cold carcass weight and yield (24.2 kg and 46.8 %), or chilling loss (2.1 %). Non-carcass components did not differ in response to the diets (P>0.05). Dietary inclusion of calcium lignosulfonate increases the proportions of udder and liver relative to empty body weight (P<0.05). Neither the cottonseed processing method nor the inclusion of calcium lignosulfonate in high-concentrate diets for cull ewes affects their performance, biometric or morphometric measurements, non-carcass components, or qualitative traits of their carcass.

The ultrastructural localization of glucose-6 dehydrogenase (G6PD) activity has been investigated using a copper ferrocyanide method in the rat liver cells by immersion fixation and freeze substitution fixation by using glutaraldehyde. After immersion in 2% glutaraldehyde fixative for 30min at 4•Ž, the site of reaction products, copper ferrocyanide, were on the cytoplasm close to the r-ER in hepatic parenchymal cells of rat. These findings were ascertained by several control experiments and X-ray microanalysis. After the metal contact quenching at-196•Ž, freeze substitution fixation, 2% glutaraldehyde in aceton for 3 days at-80•Ž, was performed. The reaction products were not observed on the cytoplasm close to the r-ER, but also on the cytoplasm near the r-ER. It is suggested that freeze substitution fixation were useful to elucidate the ultrastructural sites of soluble enzyme. study of basic FGF related to the development of capillaries in the bovine retina. Hitoshi ISHIGOOKA (1) and Takashi KITAOKA (2)

The agricultural activity has generated a progressive amount of waste, which needs a proper treatment to avoid negative environmental impacts. At the same time, values can be added to such waste, as its use in animal feed. This research was conducted at the laboratory of Animal Nutrition, State University of Southwestern Bahia, campuses of Vitória da Conquista and Itapetinga. The objective of this study was to evaluate the effect of coffee husks on ruminant feeds by increasing doses of fibrolytic enzymes, evaluating their effects on in vitro ruminal degradability of dry matter (DM), neutral detergent fiber (NDF), and acid detergent fiber (FDA) of the coffee husk (CH). The experiment was a completely randomized design in a 2x4 factorial scheme. It compounded the following treatments: Coffee husk (CH1): 1.5% enzymes (E) and 24 h enzymatic action (EA); CH2: 3.0% (E) and 24h (EA); CH3: 4.5% (E) and 24 h (EA); CH4: 6% (E) and 24 h (EA); CH5: 1.5% (E) 48 h (EA); CH6: 3% (E) and 48h (EA); CH7: 4.5% (E) and 48h (EA); and CH8: 6% (E) and 48 h (EA), all based on dry matter. An improvement in the degradability of the nutritional parameters MS, NDF, and FDA occurred with the addition of enzymes, with 3% enzyme addition being the best level, and 24 hours, as the best action time. In addition to that, as the EA on coffee husk increased, the degradation rate decreased. Therefore, the use of enzymes can improve the digestibility of the fibrous fraction, enabling the use of the coffee husk and possibly other agroindustrial residues, thus minimizing their adverse effects on nature. ResumoA atividade agrícola tem gerado uma quantidade progressiva de resíduos, os quais necessitam serem tratados de forma adequada para que não promovam impactos ambientais negativos e que, ao mesmo tempo, sejam agregados valores a estes para que possam ser utilizados na alimentação animal. A pesquisa foi conduzida no laboratório de Nutrição Animal da Universidade Estadual do Sudoeste da Bahia, campus de Vitória da Conquista e Itapetinga. Objetivou-se o aproveitamento da casca de café na alimentação de ruminantes através do tratamento com doses crescentes de enzimas fibrolíticas avaliando seus efeitos sobre a degradabilidade ruminal in vitro da matéria seca (MS), fibra em detergente neutro (FDN) e fibra em detergente ácido (FDA) da casca de café (CC). O delineamento experimental utilizado foi inteiramente casualisado, com esquema fatorial 2x4, constituindo os seguintes tratamentos Casca de café (CC1): 1,5% de Enzimas (E) e 24h de Ação Enzimática (AE); CC2: 3% (E) e 24h (AE); CC3: 4,5%(E) e 24 h(AE); CC4:6 % (E) e 24 h(AE); CC5: 1,5% (E) 48 h(AE); CC6: 3% (E) e 48h(AE); CC7:4,5% (E) e 48h(AE); CC8: 6% (E) e 48 h(AE), com base na matéria seca. Constatou-se que com adição de enzimas, ocorreu uma melhora na degradabilidade dos parâmetros nutricionais MS, FDN e FDA, sendo 3% de enzimas o melhor nível e 24 horas o melhor tempo de ação. Além disso, à medida que a ação enzimática na casca de café aumentou, a taxa de degradação diminuiu. Portanto, o us...