Background: Many pharmacological substances are known to cause hepatic injuries and paracetamol is one out of them. This study was carried out to investigate the hepatoprotective and antioxidant activity of hydroalcoholic extract of Murraya koenigii leaves in paracetamol induced hepatotoxicity in Rats.Methods: Experimental animal used in this study were 30 healthy male albino Wistar rats of 10 to 12 wks weighing 180 ± 20 g. After acclimatization for a period of one week, the rats were randomized into five groups of six rats each. Safety profile and dose selection of extract was evaluated using acute toxicity studies. Five groups named as Normal control, Paracetamol induced hepatotoxicity, Murraya koenigii leaves extract 100 mg/kg bw, Murraya koenigii leaves extract 200 mg/kg bw and Silymarin group respectively. The doses of drugs and plant extract was calculated based on the body weight of each animal and administered orally for 7 days. On 8th day rats were sacrificed and blood samples were collected by cardiac puncture for biochemical estimation of biochemical parameters. Then abdomen was opened to get liver sample for antioxidant activity and histopathology.Results: Acute toxicity studies showed the non-toxic nature of Murraya koenigii leaves extract upto dose of 2000 mg/kg body weight. Murraya koenigii leaves extract in both doses showed a significant drop in the mean levels of AST, ALT, ALP, TP and TB when compared with toxic control group. The higher dose was found better than lower dose. Silymarin was found better than both the doses. Murraya koenigii leaves extract in both doses significantly reduced the TBARS level when compared to toxic control group. The activities of GSH, SOD and CAT in liver were significantly lower in Paracetamol induced hepatotoxicity rats compared to control rats. Murraya koenigii leaves extract at both doses showed a significant increase in GSH, SOD and CAT. The higher dose was found better than lower dose. Silymarin was found better than both the doses. Histopathology of Liver biopsy with higher dose of Murraya koenigii leaves extract showed reduced periportal inflammation with mild hepatic venous congestion and Silymarin treated rats showed no periportal inflammation with mild congestion in few central veins.Conclusions: Murraya koenigii leaves extract possesses significant Hepatoprotective property; this may be due to antioxidant activity. Further studies are required to determine the exact mechanism.

Objective: The objective of the study was to investigate the analgesic activity of hydroalcoholic extract of Murraya koenigii and Coriandrum sativum leaves and compared it with standard drug in an animal model. Methods:Hydroalcoholic extracts of M. koenigii and C. sativum leaves were obtained using Soxhlet apparatus. The central analgesic property was screened by hot plate method in mice and tail flick method in rats. The pain reaction time (PRT) was measured at 30, 60, and 120 min. The peripheral analgesic activity was evaluated by acetic acid induced writhing in mice. Results:In hot plate method M. koenigii leaves extract at both doses and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. C. sativum leaves extract showed significant increase in PRT only at 60 and 120 min compared to control group. In tail flick method M. koenigii leaves extract at both doses, higher dose of C. sativum leaves extract and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. Higher dose of M. koenigii leaves extract (200 mg/kg) was comparable with standard drug tramadol in both the methods. M. koenigii leaves extract at both dose showed significant reduction in the number of writhing but C. sativum leaves extract failed to show any significant reduction in the number of writhing compared with control. Higher dose of M. koenigii leaves extract was comparable with standard drug tramadol. Conclusion:M. koenigii leaves extract showed both peripheral and central analgesic effect while C. sativum leaves extract showed only peripheral analgesic effect.

Objective: The objective of the study was to investigate the analgesic activity of hydroalcoholic extract of Murraya koenigii and Coriandrum sativum leaves and compared it with standard drug in an animal model. Methods:Hydroalcoholic extracts of M. koenigii and C. sativum leaves were obtained using Soxhlet apparatus. The central analgesic property was screened by hot plate method in mice and tail flick method in rats. The pain reaction time (PRT) was measured at 30, 60, and 120 min. The peripheral analgesic activity was evaluated by acetic acid induced writhing in mice. Results:In hot plate method M. koenigii leaves extract at both doses and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. C. sativum leaves extract showed significant increase in PRT only at 60 and 120 min compared to control group. In tail flick method M. koenigii leaves extract at both doses, higher dose of C. sativum leaves extract and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. Higher dose of M. koenigii leaves extract (200 mg/kg) was comparable with standard drug tramadol in both the methods. M. koenigii leaves extract at both dose showed significant reduction in the number of writhing but C. sativum leaves extract failed to show any significant reduction in the number of writhing compared with control. Higher dose of M. koenigii leaves extract was comparable with standard drug tramadol. Conclusion:M. koenigii leaves extract showed both peripheral and central analgesic effect while C. sativum leaves extract showed only peripheral analgesic effect.