Hepatoprotective and antioxidant activity of Murraya Koenigii leaves extract against paracetamol induced hepatotoxicity in Rats

Salwe, Kartik J; R, Mirunalini; Mano, Jervin; Manimekalai, K

doi:10.18203/2319-2003.ijbcp20172044

Cited by 8 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased ALP synthesis in response to rising biliary pressure may be the cause of the elevated ALP serum level. [17][18][19][20] In the present study, a tested acute oral APAP dose is capable to elevate ALT, AST, and ALP activities in the blood (significant elevation) compared to the control animals group, which may be an indication of liver damage and cell necrosis resulting from the formation of NAPQI over GSH detoxification capacity. 17,19 A few earlier studies have shown the same findings.…”

Section: Discussionmentioning

confidence: 53%

The Potential Effect of Silymarin Against Paracetamol-Induced Hepatotoxicity in Male Albino Rats

Abed¹,

Khalaf²,

Alnori³

2022

Pharmacogn J.

View full text Add to dashboard Cite

Background: Being the main metabolic organ, liver stays in touch with toxicity of introduced materials including, drugs. Protection is priceless to avoid complication of liver toxicity. Objectives: This research aimed to assess the protective impact of silymarin (SIL) on hepatotoxicity based on acute paracetamol (APAP) intoxication in rats in comparison with N-acetylcysteine (NAC). Methods: To do so serum was collected and the liver was analyzed for histological findings on rat model-paracetamol toxicity whether alone or in combination with SIL or NAC. The scenario was based on either preconditioning with SIL/NAC before induction of toxicity or afterwards. Serum liver function tests, pro-oxidant/antioxidant status, and proinflammatory markers were detected alongside liver histological study. Results: The results showed that liver function indices, oxidative state, and pro-inflammatory parameters were significantly changed, and histopathological alterations were detected in the liver of the intoxicated group. These modifications were inverted in groups treated with either SIL or NAC. The results of the current study suggested that SIL might be employed as a hepatoprotective drug against liver damage induced by APAP because of its ability to reduce lipid peroxidation, improve antioxidant defense status, and have anti-inflammatory effects. Conclusion: These results are equivalent to NAC therapy which is a standard drug against APAPrelated hepatotoxicity.

show abstract

Section: Discussionmentioning

confidence: 53%

The Potential Effect of Silymarin Against Paracetamol-Induced Hepatotoxicity in Male Albino Rats

Abed¹,

Khalaf²,

Alnori³

2022

Pharmacogn J.

View full text Add to dashboard Cite

show abstract

“…Alkaloids are the majority of compounds that presences in this sample. Leaves of M. koenigii contained alkaloids and tannins (Salwe et al, 2017), alkaloids, terpenoids and flavonoids (Patil, 2018), alkaloids, saponins, tannins, andflavonoids (Widyanti et al, 2019), alkaloids and terpenoids (Mustanir et al, 2019), alkaloids (Samantha el al., 2018 and terpenoids and polyphenols (Tan et al, 2017)…”

Section: Results and Discussion Separation Compound Using Column Chromatographymentioning

confidence: 99%

Evaluation of Antioxidant Activities From Ethyl Acetate Fraction of Curry Leaf Using DPPH Method

Amna

Halimatussakdiah

Ihsan

et al. 2021

EKW

View full text Add to dashboard Cite

Curry Plant (Murraya koenigii (Linn.) Spreng) is one of the plants that thrive in Indonesia. Curry contains secondary metabolites of alkaloids and flavonoids that have the potential as antioxidants. Antioxidants can reduce the activity of free radicals that can cause degenerative diseases such as cancer, coronary heart disease, and premature ageing of body cells by donating the protons to free radical compounds. This study aimed to determine the antioxidant activity and identify the active compound class of ethyl acetate fraction from curry leaves. Separation of secondary metabolites of ethyl acetate extracts was carried out by column chromatography method, which obtained 5 different fractions. The antioxidant activity of fractions A, B, C, D, and E was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with variations concentrations of 25, 50, 100, 200, and 400 ppm, and ascorbic acid was used as a comparison which measured at a wavelength of 517 nm. The results showed that fractions A and E have weak antioxidant activities, while fractions B, C, and D showed moderate antioxidant activities. The highest antioxidant activity was found in fraction B with an IC50 value of 341.38 ppm, where the fraction showed the presence of secondary metabolites of alkaloids and terpenoids.Abstrak: Tanaman kari ((Murraya koenigii (Linn.) Spreng) merupakan salah satu tanaman yang tersebar di Indonesia. Kari mengandung senyawa metabolit sekunder alkaloid dan flavonoid yang memiliki potensi sebagai antioksidan. Antioksidan dapat mengurangi aktivitas radikal bebas yang dapat menyebabkan penyakit degeneratif seperti kanker, penyakit jantung coroner dan penuaan sel dengan cara menyumbangkan proton pada senyawa radikal bebas. Tujuan dari penelitian ini adalah untuk menentukan aktivitas antioksidan dan mengidentifikasi kelas senyawa aktif fraksi etil asetat dari daun kari. Pemisahan metabolit sekunder dari ekstrak etil asetat dilakukan menggunakan metode kromatografi kolom dengan menghasilkan 5 fraksi yang berbeda. Aktivitas antioksidan dari fraksi A, B, C ,D ,dan E dilakukan menggunakan metode DPPH (1,1-diphenil-2-pikrilhydrazil) dengan variasi konsentrasi 25, 50, 100, 200, dan 400 ppm dan asam askorbat digunakan sebagai perbandingan yang diukur pada panjang gelombang 517 nm. Hasil menunjukkan bahwa fraksi A dan E memiliki aktivitas antioksidan yang lemah, sedangkan fraksi B, C, dan D menunjukkan aktivitas antioksidan sedang. Aktivitas antioksidan yang paling tinggi ditemukan pada fraksi B dengan IC50 341,38 ppm. Fraksi tersebut menunjukkan adanya kandungan senyawa metabolit sekunder alkaloid dan terpenoid.

show abstract

“…The decreased lethality effect of the extracts observed in the present study led to subsequent investigations carried out to confirm further hepatoprotective effects. The optimum dose of APAP (2 g/kg), which induced the liver damage, was determined to be 2 g/kg through the selection of three doses from previous studies, which was a similar procedure used in other studies [ 28 , 39 ]. In the present study, administration of 2 g/kg APAP resulted in significant elevation of serum levels ALT, AST, ALP, TB, lipid profiles, LDL, TC, and TGs and decreased serum level of HDL, which was in agreement with the previous study [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effects of Crude Stem Bark Extracts and Solvent Fractions of Cordia africana against Acetaminophen-Induced Liver Injury in Rats

Geresu

Umer

Arayaselassie

et al. 2022

Canadian Journal of Gastroenterology and Hepatology

View full text Add to dashboard Cite

Cordia africana Lam (Boraginaceae) is widely used in Ethiopian folk medicine for the treatment of different types of liver disorders. Thus, this study aimed to investigate the hepatoprotective effects of an aqueous (CAAE), 80% methanol extracts of C. africana stem bark (CAME), and the solvent fractions of the methanol extract against acetaminophen (APAP)-induced liver injury in rats. Acute toxicity test and APAP-induced lethality test were done on mice of either sex, while APAP dose selection test was done on female rats. Male rats were used for hepatoprotective experiments and the liver injury was induced using 2 g/kg APAP given orally. Serum levels of the liver enzymes and total bilirubin (TB), as well as lipid profiles, were determined. Histopathological examination of the liver tissues was also conducted to confirm the findings of biochemical analysis. Intraperitoneal (i.p.) sodium pentobarbital (SPB)-induced sleeping duration was also used to determine the protective effect of the test substances. Oral administration of APAP resulted in a significant increase in serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), TB, low-density lipoprotein (LDL), total cholesterol (TC), and triglycerides (TGs) and decrease in serum high-density lipoprotein (HDL). Administration of the standard drug, silymarin 100 mg/kg, extracts at doses of 100, 200, and 400 mg/kg and fractions at the dose of 400 mg/kg reversed the serum levels of all parameters to normal. CAME exerted a significant dose-dependent hepatoprotective effect in terms of ALT and AST, while CAAE significant dose-dependent hepatoprotective effect was in terms of AST, ALP, and TGs. The protective effect of the extracts and fractions was also confirmed by histopathological investigations and SPB-induced sleeping time. From the results of the present study, it can be concluded that C. africana stem bark aqueous, 80% methanol crude extracts, and solvent fractions of the methanol extract showed hepatoprotective effects.

show abstract

Hepatoprotective and antioxidant activity of Murraya Koenigii leaves extract against paracetamol induced hepatotoxicity in Rats

Cited by 8 publications

References 18 publications

The Potential Effect of Silymarin Against Paracetamol-Induced Hepatotoxicity in Male Albino Rats

The Potential Effect of Silymarin Against Paracetamol-Induced Hepatotoxicity in Male Albino Rats

Evaluation of Antioxidant Activities From Ethyl Acetate Fraction of Curry Leaf Using DPPH Method

Hepatoprotective Effects of Crude Stem Bark Extracts and Solvent Fractions of Cordia africana against Acetaminophen-Induced Liver Injury in Rats

Contact Info

Product

Resources

About