Chromosomes, sub-chromosomal regions and genes are repositioned during cell differentiation to acquire a cell-type-specific spatial organization. The constraints that are responsible for this cell-type-specific spatial genome positioning are unknown. In this study we addressed the question of whether epigenetic genome modifications may represent constraints to the acquisition of a specific nuclear organization. The organization of kinetochores, pericentric heterochromatin and the nucleolus was analysed in pre-implantation mouse embryos obtained by in-vitro fertilization (IVF), parthenogenetic activation (P) and nuclear transfer (NT) of differentiated somatic nuclei, which possess different epigenomes. Each stage of pre-implantation embryonic development is characterized by a stage-specific spatial organization of nucleoli, kinetochores and pericentric heterochromatin. Despite differences in the frequencies and the time-course of nuclear architecture reprogramming events, by the eight-cell stage P and NT embryos achieved the same distinct nuclear organization in the majority of embryos as observed for IVF embryos. At this stage the gametic or somatic nuclear architecture of IVF or P and NT embryos, respectively, is replaced by a common embryonic nuclear architecture. This finding suggests that the epigenome of the three types of embryos partially acts as a constraint of the nuclear organization of the three nuclear subcompartments analysed.

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Cognitive rigidity and behavioral and communication problems are the most common symptoms that characterize Autism Spectrum Disorder (ASD). In fact, people with ASD face complications in everyday life, from interacting with other persons to being autonomous in the most ordinary daily tasks. To overcome these limitations and improve their quality of life, we developed 5A. 5A exploits cutting-edge technologies such as Virtual Reality (VR), Augmented Reality (AR), and Conversational Agents (CA), combining smartphones and wearable headsets to offer new forms of intervention to persons with ASD. Virtual reality experiences enable learning common tasks, such as using public transport, through simulation in a safe environment. When people are in similar reallife situations, AR can help them generalize the skills they have learned in VR by overlaying interactive media assets on top of the view of the physical world around them. A customizable conversational assistant provides individualized prompts and feedback in both VR and AR to provide maximum user support. CCS CONCEPTS• Human-centered computing → Virtual reality; Mixed / augmented reality.

Measuring cognitive functions is a complex and challenging process, and researchers usually conduct their study in experimental settings and with standardized tests. Such an approach is raising more and more criticism. Especially about the capability of psychology's laboratory experiments to provide generalizable and accurate indicators of impairments outside the laboratory boundaries in everyday life. This is acknowledged as the "real-world or lab" dilemma. Most tests traditionally performed using paper-and-pencil may fail to detect the individual's difficulties in the real world. The scientific community has launched a quest for new digitized, interactive, and more "ecological" versions. Our work investigates a novel approach to this topic that exploits interactive Multi-Sensory Environments (iMSE) and questions how iMSEs could contribute to the neuropsychology assessment field. The paper describes NEP-Neuro-Psychological Suite, a set of game-based activities in iMSE inspired by widely adopted neuropsychological tests. The suite provides a context in which existing or new neuropsychological tests can be experimented, extended, and modified with stimuli, contents, and tasks closer to real-world situations. We report an exploratory empirical study (N=22) on a well-known test to assess attention skills, the Stroop Test. The results, although very preliminary, provide insights into the effects of transposing classic tests into a novel form and the role that iMSEs can play in the debate about the "real-world or lab" dilemma.

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