The colonization of the human gut microbiome begins at birth, and over time, these microbial communities become increasingly complex. Most of what we currently know about the human microbiome, especially in early stages of development, was described using culture-independent sequencing methods that allow us to identify the taxonomic composition of microbial communities using genomic techniques, such as amplicon or shotgun metagenomic sequencing. Each method has distinct tradeoffs, but there has not been a direct comparison of the utility of these methods in stool samples from very young children, which have different features than those of adults. We compared the effects of profiling the human infant gut microbiome with 16S rRNA amplicon vs. shotgun metagenomic sequencing techniques in 338 fecal samples; younger than 15, 15–30, and older than 30 months of age. We demonstrate that observed changes in alpha-diversity and beta-diversity with age occur to similar extents using both profiling methods. We also show that 16S rRNA profiling identified a larger number of genera and we find several genera that are missed or underrepresented by each profiling method. We present the link between alpha diversity and shotgun metagenomic sequencing depth for children of different ages. These findings provide a guide for selecting an appropriate method and sequencing depth for the three studied age groups.

This study aimed to identify sociodemographic characteristics of university students in the last year in the health field, to verify whether they have Common Mental Disorders (CMD) and to investigate the relationship between CMD and the perception of stress and self esteem in this population. This is a quantitative, descriptive and exploratory study, involving students from biomedicine, nursing, nutrition, physical education, pharmacy, physiotherapy and psychology courses, with a sample of 184 university students. The Sociodemographic questionnaire, the Self-Reporting Questionnaire (SRQ-20), the Perceived Stress Scale PSS-14 and the Rosenberg Self Esteem Scale (EAR) were used for data collection. The analysis was performed using the software Statistical Package for the Social Sciences SPSS® version 21, with the adoption of a significance level of 5% (alpha = 0.05) in all analysis. As a result, CMD were present in 116 (63.0%) students. There was a statistically significant association between CMD and stress and between CMD and self esteem (p <0.001), which expresses an extremely significant result. In fact, the expressive prevalence of CMD, associated with the levels of stress and self esteem identified in this study, reveal the immediate need for the insertion of actions and projects by the university, aimed at the well-being of the university population and health promotion mental, considering the scenario prone to illness and the vulnerability of students.

This study aimed to assess the association between personal and environmental factors with recent stress in nursing students. This is a cross-sectional study with a quantitative approach carried out with 209 nursing students. Data were collected using a Sociodemographic Questionnaire, Inventory of Symptoms of Stress (ISSL) for recent stress, Questionnaire on Trauma in Childhood (CTQ), Perceived Stress Scale (PSS-14) and Rosenberg's Self-Esteem Scale. Among the students, 163 (78%) had recent stress, the majority in the resistance phase 120 (57.4%) and with manifestations of psychological symptoms 121 (57.9%). Recent stress was associated with health problems, medication use, self-reported stress, perceived stress and demands for social readjustment. The identification of factors that predispose to recent stress is essential for planning interventions focused on protecting the health of nursing students.

The aim of this study was to verify through personal and environmental factors which are the predictors of recent stress in nursing students. This is a cross-sectional study with a quantitative approach. For data collection, we used Sociodemographic Questionnaire, Stress Symptom Inventory (ISSL) for recent stress, Childhood Trauma Questionnaire (CTQ), Perceived Stress Scale (PSS-14) and Rosenberg Self-Esteem Scale. To verify the predictor variables of recent stress, the statistical analysis used binary logistic regression. The study was attended by 209 college students. Physical activity, smoking, high self-esteem, early stress and religion were pointed as predictors of recent stress. Average and high perception of stress, being married, working, working in health care, weekly leisure activities represented lower chances for stress. The identification of stress predictor variables helps in the elaboration of the profile of college students most vulnerable to stress.

O presente estudo teve como objetivo investigar a aplicabilidade da resiliência como fator de proteção junto às atividades dos enfermeiros. Trata-se de um estudo descritivo e exploratório realizado em um hospital filantrópico de pequeno porte localizado na cidade de Auriflama/SP, com 10 enfermeiros alocados no hospital. A coleta de dados deu-se por meio de entrevista áudio gravada, onde as respostas foram transcritas e agrupadas por semelhanças. Identificou-se 3 categorias, sendo, o conhecimento acerca do termo resiliência, fatores relacionados à vulnerabilidade ocupacional e fortalezas nas adversidades diárias. Consubstanciando-se nos resultados encontrados, foi possível verificar que os participantes possuem uma noção superficial do termo, apresentam esgotamento profissional, jornada de trabalho exaustiva, que o ambiente laboral favorece o adoecimento e para que a resiliência esteja no ambiente de trabalho é necessário o equilíbrio entre fatores de risco e de proteção.