Several CPGs for SAP were developed without the desired methodological rigour or transparency. Synthesis of recommendations for best-evaluated CPGs provides a broad approach owing to the complementarity of the recommendations.

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BACKGROUND: Lectins from Moringa oleifera seeds (WSMoL), Myracrodruon urundeuva bark (MuBL), and heartwood (MuHL) are larvicidal agents against Aedes aegypti; in addition, WSMoL is an ovicidal agent against this mosquito. In this work, we evaluated the ovicidal activity of MuBL and MuHL by determining the concentrations that reduce the hatching rates by 50% in 72 h (EC 50 ). The effects of WSMoL, MuBL, and MuHL on the ultrastructure of the eggs' surface were assessed by scanning electron microscopy (SEM). In addition, the ability of these lectins to penetrate the eggs was investigated by using conjugates of the lectins with fluorescein isothiocyanate (FITC). RESULTS: MuBL andMuHL were ovicidal agents with EC 50 of 0.26 and 0.80 mg/mL (260 and 800 ppm), respectively. SEM images of eggs treated with WSMoL for 24 h revealed discontinuity of the exochorionic network and the absence of the exochorionic cells and their tubercles. After 48 and 72 h of incubation, strong deformation and degeneration of egg surfaces were observed. In MuBL and MuHL-treated eggs, the presence of lumps on the surface of the eggs, disappearance of the exochorionic network and the decrease and deformation of tubercles were observed. Lastly, fluorescence microscopy revealed that the three lectins were able to enter the eggs and reach the digestive tract of the embryos. CONCLUSION: WSMoL, MuBL, and MuHL are ovicidal agents on A. aegypti that have differing efficiencies in terms of how they cause alterations in the chorionic surface and in terms of their ability to penetrate the eggs.

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