Root-knot nematodes (Meloidogyne spp.) cause great losses in tomato crops. An environmentally friendly for its control is the use of predatory fungi such as Purporeocillum lilacinum, which reduces its population in the soil and mitigates yield losses. Therefore, the objective of the present study was to evaluate the biocontrol efficacy of the strains of the nematophagous fungus Purporeocillum lilacinum and liquid bioformulates on the eggs of the root-knot nematode Meloidogyne incognita, and the formation of galls on the tomato root. Two native strains of Purporeocillum lilacinum H2 and H3 combined with the bioformulated Extract of Beneficial Microorganisms (EPMB®) and the root exudate stimulator Exu-Root® were tested on Meloidogyne incognita eggs and tomato plants. The results obtained indicate that the Purporeocillum lilacinum H2 and H3 strains infected the Meloidogyne incognita eggs, interrupted the development of the embryos and caused their death, which significantly reduced the presence of galls in the root of the plants. In short, the maximum biological performance was presented with the treatments H2+Exu-Root® and H3+EPMB®, which had the lowest number of galls with 19.2 and 20.3 galls per plant respectively, compared to the control that presented 88 galls in the root and the treatments where the fungus was not applied (69-85 galls). Finally, it is concluded that the results demonstrate the potential of the Purporeocillum lilacinum H2 and H3 strains as biocontrol agents against the root-knot nematode Meloidogyne incognita, and that, in combination with EPMB® and Exu-Root®, the efficacy can be increased to reduce its population.
The objective was to evaluate and compare the precision of estimation of dasometric variables, survey times and costs of two sampling methods versus a census, applied to a natural even-age mass of Pinus arizonica Engelm. in the municipality of Guachochi, Chihuahua, Mexico. Nine fixed-dimension and nine variable-dimension sites were performed with Bitterlich's angular sampling at coincident sample points. Times from start to finish were taken by site and technicians from the State were surveyed to determine sampling costs. ANOVA tests were developed at a significance level of 0.05, comparing both methods with the census. The results indicate that, in both samplings, the number of trees per hectare does not present statistical differences with respect to the census. The basal area by both methods was not statistically different, however, fixed dimensions present an error greater than 5%. Finally, the volume per hectare estimated was similar to that of the census, according to the rates carried out, greater precision was found by the variable area method (error=0.03%). The execution time showed significant differences (p=0.0001), the fixed dimension site required a mean time of 44 minutes and the variable dimension one 10 minutes 26 seconds. The total costs are 679.90 and 654.33 Euro to sample nine fixed-dimension and nine variable-dimension sites respectively, showing significant differences (p=0.0008). It is concluded that both methods are statistically acceptable for the variables evaluated in the type of forest mass studied.
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