The human kinome comprises 538 kinases playing essential functions by catalyzing protein phosphorylation. Annotation of subcellular distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 cellular compartments. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a similar condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other structures. Furthermore, KA demonstrated the role of liquid-liquid phase separation in formation of kinase condensates. Identification of MOK as a mitochondrial kinase revealed its function in cristae dynamics, respiration, and oxidative stress response. Although limited by possible mislocalization due to overexpression or epitope tagging, this subcellular map of the kinome can be used to refine regulatory mechanisms involving protein phosphorylation.

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Background Granulomatous capillary haemangioma refers to a benign vascular tumour that commonly affects the skin, with occasional involvement of the mucosa. Reports of conjunctival granulomatous capillary haemangioma in children are uncommon. In this article, we present a case of granulomatous capillary haemangioma and a brief review of the relevant literature. Case presentation An 11-year-old girl presented with a conjunctival mass. An excision of the entire lesion was performed. Histopathology showed a granulomatous capillary haemangioma. Conclusions The clinical manifestations of granulomatous capillary haemangioma lack specificity; pathological characteristics and immunohistochemistry are the main basis for diagnosis. We retrospectively analysed the diagnosis and treatment of a patient with conjunctival granulomatous capillary haemangioma to deepen the understanding and facilitate the diagnosis and treatment of this disease.

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Background: Orbital lymphoproliferative disorders (OLPDs) consist of a spectrum of diseases ranging from benign to malignant lesions including reactive lymphoid hyperplasia, atypical lymphoid hyperplasia, and lymphoma. OLPDs rarely present as an orbital mass lesion in children. Accurate discrimination of OLPDs is crucial for treatment planning. We report a case to investigate the clinical and pathological features of OLPDs in children. Case presentation: A 3-year-old female with orbital mass was admitted to the hospital and proceeded to have a CT orbit which showed an orbital mass. The orbital mass was removed after operation and pathologic diagnosis was identified. Pathological diagnosis with histopathological features and immunohistochemical markers was lymphoproliferative lesion in the left orbit. The diagnosis was consistent with OLPDs. There was no recurrence after one-year follow-up. Conclusions: OLPDs are rare in children. The clinical manifestations and imaging have no specificity; pathological diagnosis with histopathological features and immunohistochemical markers is the main basis for the diagnosis and treatment.