Holant problem is a general framework to study the computational complexity of counting problems. We prove a complexity dichotomy theorem for Holant problems over the Boolean domain with non-negative weights. It is the first complete Holant dichotomy where constraint functions are not necessarily symmetric.Holant problems are indeed read-twice #CSPs. Intuitively, some #CSPs that are #P-hard become tractable when restricted to read-twice instances. To capture them, we introduce the Block-rank-one condition. It turns out that the condition leads to a clear separation. If a function set F satisfies the condition, then F is of affine type or product type. Otherwise (a) Holant(F) is #P-hard; or (b) every function in F is a tensor product of functions of arity at most 2; or (c) F is transformable to a product type by some real orthogonal matrix. Holographic transformations play an important role in both the hardness proof and the characterization of tractability.

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