Terahertz technology has shown broad prospects for measuring corneal water content, which is an important parameter of ocular health. Based on terahertz time-domain spectroscopy, a new indicator named characteristic ratio (CR) of the sum of low (0.2-0.7 THz) and high (0.7-1.0 THz) frequency spectral intensities, for characterizing corneal hydration is introduced in this work. CR is calculated from the real-time reflection spectra after error elimination of ex vivo human corneal stroma samples which is collected during dehydration under natural conditions (temperature: 22.4 ± 0.3°C; humidity: 20.0 ± 3%). The corresponding relationships between CR and corneal water content are reported. Comparing the linear fitting results with the published similar study, the coefficients of variation of the fitting slope and intercept are 39.4% and 27.6% lower, respectively. This indicates that this approach has the potential to achieve corneal water content in-vivo detection in the future.

Corneal water content and hydrodynamics are critical indicators of eye health. In this work, a convenient method based on near‐infrared absorption spectroscopy (NIRA) was presented to measure the relative water content of the corneal stroma ex vivo, which paves the way to measure corneal water content in vivo. The relative water content of fresh corneal stroma during dehydration under natural conditions (temperature, 25.8 ± 0.3°C; humidity, 7.2% ± 0.9%) was monitored in real time, and the characteristic time τ when the relative water content dropped to 90% of the fresh corneal stroma was 140.1 ± 30.6 s. Furthermore, the change in the relative water content over time was found to be linear with a dehydration rate of 0.071% per second, consistent with indirect optical coherence pachymetry but with superior reproducibility and precision. Provided that the NIRA spectrometer is changed to a reflection structure from the current transmission configuration, the NIRA method proposed in this work has great potential for in vivo measurement with the advantages of non‐contact, high precision and low cost.

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