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Background: Long noncoding RNA (lncRNA) plays a critical role in initiating lung cancer. This study aims to research the function and mechanism of lncRNA HIF1A-AS2 in regulating non-small cell lung cancer (NSCLC) progression. Methods: qRT-PCR was used to analyze gene expression. The CCK-8 assay was performed to detect cell proliferation. The Transwell assay was conducted to examine cell migration and invasion. A Caspase3 activity detection kit was utilized to analyze apoptosis. The luciferase reporter assay was carried out to research interactions of HIF1A-AS2, miR-153-5p and S100A14. Results: HIF1A-AS2 expression was raised in NSCLC tissues and cell lines. The HIF1A-AS2 level was increased in advanced NSCLC tumor tissues. High HIF1A-AS2 expression was related to poor prognosis. HIF1A-AS2 knockdown decreased proliferation, migration and invasion while promoting apoptosis. HIF1A-AS2 was the sponge for miR-153-5p, and miR-153-5p targeted S100A14. HIF1A-AS2 promoted S100A14 expression through regulating miR-153-5p. Conclusion: The HIF1A-AS2/miR-153-5p/S100A14 axis plays a crucial role in promoting NSCLC progression.

Genetic parameters and genotype by rearing density interactions for growth traits and survival were investigated in Litopenaeus vannamei. A total of 3626 shrimps from 40 families in two densities (low density, 80 shrimps m À2 ; high density, 160 shrimps m À2 ) were measured for four growth traits, body length, body weight, cephalothorax length and abdominal segment length. Variance components and genetic parameters for growth and survival were estimated using a two-trait animal model or a threshold (probit) sire and dam model. The common environment effect was excluded because it could not be partitioned effectively, likely because of high heritability. The heritability for growth traits varied from 0.21 to 0.44, indicating a moderate level. For survival, the backtransformed heritability (h 2 p ) was 0.36 AE 0.06 and 0.22 AE 0.04 for the low and high densities respectively. There were no significant differences in heritability for growth traits and survival between the two densities (P > 0.05). High genetic correlations (0.94-0.98) between the two densities for growth traits indicated a low genotype by density interaction and ranking effect across the environments, which suggested that these traits were controlled mostly by the same genes over the two densities. Therefore, selection in a low-density environment should produce desired correlated responses for growth at a high density. Genetic correlation (0.77 AE 0.09) between the two densities for survival was moderate and significant, which indicated that the genotype by density interaction effect was moderate and low for survival under the two densities. Our results provide crucial information for use in L. vannamei breeding programmes in China.