The utilization of intelligent controlled release technology to create stimuli-responsive pesticide formulations has been shown to effectively improve pesticide efficacy and reduce environmental pollution. Herein, a glutathione-responsive release polyurea (PU) microcapsules (MCs) loaded with pyraclostrobin were developed via the interface polymerization method. The pyraclostrobin-loaded PU-MCs showed a regular spherical shape with an average diameter of 480 nm. It also showed good thermal stability and rheological properties. Furthermore, the pyraclostrobin-loaded PU-MCs exhibited favorable wettability on wheat leaves, which was beneficial for enhancing the retention capacity of pesticide droplets and improving pesticide utilization. The pyraclostrobin can be released from MCs and directly proportional to glutathione (GSH) concentrations with Fickian diffusion. Importantly, the control efficacy of pyraclostrobin-loaded PU-MCs against Fusarium graminearum was positively correlated with GSH, indicating a promising candidate for a controlled release of pesticides in agriculture and laying the foundation for further field experiments.

Leucine aminopeptidase (LAP), as a vital proteolytic enzyme, its overexpression is often associated with many physiological diseases, such as liver dysfunction and breast cancer. Therefore, the accurate measurement of LAP...

Hydrogen sulfide (H2S) is one of the typical reactive sulfur species, which exhibits an important role in regulating both physiological and pathological processes. Recent studies indicate that H2S also serves as a key signaling molecule in a broad range of regulatory processes in plants. However, in situ imaging and detection of the levels of H2S in plant tissues remains a challenge. In this work, a NIR fluorescent probe (HBTP-H 2 S) was synthesized to achieve H2S imaging in living plant tissues. HBTP-H 2 S exhibited high sensitivity toward H2S with a large Stokes shift (250 nm). HBTP-H 2 S could be applied to HeLa cells to detect the fluctuation of endogenous H2S levels in response to physiological stimulations. Importantly, HBTP-H 2 S was utilized for direct H2S imaging of rice roots and revealed the upregulation of H2S signaling in response to aluminum ions and flooding stresses. Our work thus provides a new tool to investigate H2S-involved signal interaction and protective resistance of crops under environmental stresses.

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