Introduction: Osteoarthritis (OA) is a common chronic joint disease characterized by articular cartilage degeneration. OA usually manifests as joint pain, limited mobility, and joint effusion. Currently, the primary OA treatment is non-steroidal anti-inflammatory drugs (NSAIDs). Although they can alleviate the disease's clinical symptoms and signs, the drugs have some side effects. Selenium nanoparticles (SeNPs) may be an alternative to relieve OA symptoms. Materials and Results:We confirmed the anti-inflammatory effect of selenium nanoparticles (SeNPs) in vitro and in vivo experiments for OA disease in this study. In vitro experiments, we found that SeNPs could significantly reduce the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the major inflammatory factors, and had significant anti-inflammatory and anti-arthritic effects. SeNPs can inhibit reactive oxygen species (ROS) production and increased glutathione peroxidase (GPx) activity in interleukin-1beta (IL-1β)-stimulated cells. Additionally, SeNPs down-regulated matrix metalloproteinase-13 (MMP-13) and thrombospondin motifs 5 (ADAMTS-5) expressions, while up-regulated type II collagen (COL-2) and aggrecan (ACAN) expressions stimulated by IL-1β. The findings also indicated that SeNPs may exert their effects through suppressing the NF-κB p65 and p38/MAPK pathways. In vivo experiments, the prevention of OA development brought on by SeNPs was demonstrated using a DMM model. Discussion: Our results suggest that SeNPs may be a potential anti-inflammatory agent for treating OA.

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Background The indirect reduction is one kind of micro-invasive technology. The most important mechanism of indirect reduction is traction.The purpose of this study was to independently develop a traction reduction device on lower limb fracture and explore the clinical effect of micro-invasive treatment on tibial fracture adopting this device. Methods A retrospective study was conducted on 21patients with tibial fracture adopted this device to assist fracture reduction during operation from August 2019 to June 2022. The cases included 10 tibial plateau fractures, 6 tibial shaft fractures and 5 distal tibia (pilon )fractures. 3 tibial shaft fracture cases were adopted intramedullary needle internal fixations, the others cases were adopted plate internal fixations.Demographic data, fracture type, perioperative data, and radiological evaluation were collected. Clinical outcomes were graded using Rasmussen function score and American orthopedic foot and ankle society (AOFAS) score. Results The cases were followed up from 12 to 33 months.All cases had no complications such as blood vessel and nerve injuries,infection and internal fixation fail. All cases got bony healing. The mean Rasmussen anatomy score before operation of tibial plateau cases was (6.60 ± 3.13), the score immediately after operation was (17.20 ± 1.03), and there was statistical significance difference between two groups(P༜0.05). The mean Rasmussen function score of tibial plateau fracture cases at the last follow-up was(28.20 ± 1.55).The mean length difference between both lower limbs of tibia shaft fracture cases immediately after operation was (2.17 ± 2.04) mm, and the mean varus-valgus angulation of tibia shaft fracture immediately after operation was (1.67 ± 0.82)°, the mean fracture anteroposterior angulation of tibia shaft fracture immediately after operation was (2.00 ± 1.10)°. The Burwell-Charnley radiological assessment immediately after operation of distal tibia cases was as follow, 5 cases got anatomical reduction and 1 got good reduction. The mean AOFAS score of ankle and foot function at the last follow-up was( 93.60 ± 5.73). Conclusions The traction reduction device on lower limb fracture is safe effective to application, convenient to use, and has definite reduction effect. It can be applied in supine, lateral and prone positions, and be applied to micro-invasive treatment on complex tibia fracture.