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A novel glucose biosensor was fabricated. The first layer of the biosensor was polythionine, which was formed by the electrochemical polymerisation of the thionine monomer on a glassy carbon electrode. The remaining layers were coated with chitosan-MWCNTs, GOx, and the chitosan-PTFE film in sequence. The MWCNTs embedded in FAD were like “conductive wires” connecting FAD with electrode, reduced the distance between them and were propitious to fast direct electron transfer. Combining with good electrical conductivity of PTH and MWCNTs, the current response was enlarged. The sensor was a parallel multi-component reaction system (PMRS) and excellent electrocatalytic performance for glucose could be obtained without a mediator. The glucose sensor had a working voltage of −0.42 V, an optimum working temperature of 25°C, an optimum working pH of 7.0, and the best percentage of polytetrafluoroethylene emulsion (PTFE) in the outer composite film was 2%. Under the optimised conditions, the biosensor displayed a high sensitivity of 2.80 µA mM−1 cm−2 and a low detection limit of 5 µM (S/N = 3), with a response time of less than 15 s and a linear range of 0.04 mM to 2.5 mM. Furthermore, the fabricated biosensor had a good selectivity, reproducibility, and long-term stability, indicating that the novel CTS+PTFE/GOx/MWCNTs/PTH composite is a promising material for immobilization of biomolecules and fabrication of third generation biosensors.

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In order to investigate biological properties of the dominant strain from the biological activated carbon (BAC) filter column, a novel, dominant iron-and-manganese removal strain, FM-2, was screened from BAC mature biological membrane. By phylogenetic analysis based on 16S rDNA sequence comparison, FM-2 was identified to be Citrobacter freundii. The experimental results indicated that Citrobacter sp. FM-2 could remove 83.6% Fe (II) and 64.9% Mn (II) after four days. Inoculum greatly influenced the iron-manganese removal performances. The appropriate inoculum concentration was 5%. The initial concentrations of Fe (II) and Mn (II) had little negative influence on its removal performance. When Fe (II) and Mn (II) concentrations were 32.9~85.7 and 25.7~46.7 mg/L, respectively, the removal of Fe (II) and Mn (II) followed the first-order dynamical model with the velocity constants of 0.8528~0.9937/d and 0.3001~0.3179/d, respectively.