A novel itaconic acid based progesterone imprinted biosensor was prepared via a reversible addition chain transfer mechanism and surface plasmon resonance.
Reversible addition-fragmentation chain transfer (RAFT) polymerization mechanism was used for the preparation of molecularly imprinted polymers (MIPs) film using 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid (CDTPA) as chain transfer reagent and visible light initiator. In addition, the effect of CDTPA concentration on the polymerization rate was monitored, establishing that too high CDTPA concentration would inhibit the polymerization rate. The MIPs film was characterized by contact angle measurement, frontier transfer infrared spectroscopy (FTIR) and scanning electron microscope (SEM) which showed the successful grafting of MIPs films onto surface plasmon resonance (SPR) sensor chip. The response of progesterone sensing ranged from 10−16 to 10−6mol/L with low detection limit of 1× 10−16mol/L in PBS buffer (pH 7.4) and the MIPs film exhibited good selectivity, reproducibility and stability. Moreover, the sensors had been successfully applied to detect progesterone in tap water, lake water and human urine turning out with wide detection range and low detection limit.
A novel tetracycline (TC) imprinted polymer was prepared in visible light via synergy of dual functional group monomers methacrylic acid (MAA) and itaconic acid (IA) for selective detection of TC in urine and milk samples.
In this work, a hydrophilic and high sensitivity sensor was fabricated based on BMS (MAA : HEMA = 3 : 1, molar ratio) molecularly imprinted polymers (MIPs) film for 17β-estradiol (E2) detection in aqueous media combined with surface plasmon resonance (SPR) technique. In-situ UV polymerization method was used to synthesize the MIPs film on the gold surface which was modified with dodecyl mercaptan. Afterwards, the MIPs film was characterized by infrared spectroscopy (IR), scanning electron microscope (SEM) and contact angle measurements. The results showed that the MIPs film was successfully prepared on the surface of the sensor chip with good hydrophilicity and permeability. The analysis of SPR spectroscopy indicated that the MIPs film displayed greater selectivity to E2 than other competitors and non-imprinted polymers (NIPs) film and showed better adsorption performance than one kind of monomer for the same E2 concentration. The response of E2 sensor ranged from 2.5×10−16 to 2.5×10−8 mol/L with an ultra-low detection limit of 9.14×10−18mol/L in PBS buffer (pH 7.4). In addition, this sensor performed good reusability and stability. Finally, the sensor was successfully applied to detect E2 in tap water and human urine and had wide detection ranges and low detection limits in backgrounds.
Novel potassium ions imprint polymer sensor were successfully prepared by applying a facile combination of a reversible addition chain transfer mechanism (RAFT) and surface plasmon resonance (SPR). UV photopolymerization in synergy with 2-methyl-2-[(dodecylsulfanylthiocarbonyl)sulfanyl]propanoic acid (DDMAT) as a chain transfer reagent was employed for film synthesis on an SPR sensor chip for the detection of potassium ions using ethylene glycol dimethacrylate (EGDMA) as a cross linker. The modified surface of the sensor was characterized by contact angle measurements, frontier transfer infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The results of potassium ion imprint polymer film showed a high adsorption capacity and excellent selectivity in comparison to other analogues and non-imprinted polymer (NIP) film. Through 5 adsorption–desorption cycles, the high recoverability of MIP film was confirmed. Consequently, within the concentration range of 10−15-10−5 mol/L, the coupling angle change of SPR versus the negative logarithm of concentration showed excellent linearity: R2 = 0.98. Based on a linear equation, MIPs showed excellent values for the limit of detection(1.6×10−16M). Furthermore, it was also used to detect potassium ions in real sample, and in the tap water it showed high recovery and low detection limit. Hence, potassium ions imprint polymer film in combination with an SPR sensor chip demonstrated potential applications for rapid and highly effective sensing even in tap water.
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