Fabrication
of functional electrochemical biosensor is a hot topic; however, precise
and sensitive cancer detection in early clinical diagnosis is still
a great challenge. Continuous efforts have been devoted to explore
functional materials for this issue. In this work, we developed a
dual binding sites and dual signal-amplifying electrochemical aptasensor
of self-polymerized dopamine-decorated Au and coordinated with Fe-MOF
(Au@PDA@Fe-MOF) for the detection of carcinoembryonic antigen (CEA).
Remarkably, Au@PDA@Fe-MOF features high sensitivity, multiple active
sites, good biocompatibility, and excellent selectivity, which is
attributed to abundant −COOH in porous Fe-MOF and unsaturated
Fe3+ sites on the surface of Fe-MOF as the active binding
sites grafting more NH2-functionalized CEA-specific aptamer
and redox PDA and Fe-MOF accelerating the movement of electrons for
dual signal amplifying. Meanwhile, the electrochemical aptasensor
shows favorable repeatability with 1.82% relative standard deviation
(RSD) under five independent aptasensors and strong stability with
only 3.3% degradation after 12 days of storage. In addition, the aptasensor
has wide CEA detection range from 1 fg mL–1 to 1
μg mL–1 with a low detection limit of 0.33
fg mL–1 (S/N = 3). Furthermore, the aptasensor is
feasible for accurate and quantitative detection of CEA in serum samples
with RSD below 2.32%. The satisfying results demonstrate promising
applications of the CEA aptasensor in practical sample analysis and
lay an important foundation for other biomarker detection in early
clinical diagnosis.
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