The effect of stem cell transplantation in the treatment of neural lesions is so far not satisfactory. Magnetic stimulation is a feasible exogenous interference to improve transplantation outcome. However, the effect of magnetic stimulation on the differentiation of induced pluripotent stem cells (iPSCs) into neuron has not been studied. In this experiment, an in vitro neuron differentiation system from human iPSCs were established and confirmed. Three magnetic stimuli (high frequency [HF], low frequency [LF], intermittent theta‐burst stimulation [iTBS]) were applied twice a day during the differentiation process. Immunofluorescence and quantitative polymerase chain reaction (Q‐PCR) were performed to analyze the effect of magnetic stimulation. Neural stem cells were obtained on day 12, manifested as floating neurospheres expressing neural precursor markers. All groups can differentiate into neurons while glial cell markers were not detected. Both Immunofluorescence and PCR results showed LF and iTBS increased the transcription and expression of neuronal nuclei (NeuN). HF significantly increased vesicular glutamate transporters2 transcription while iTBS promoted transcription of both synaptophysin and postsynaptic density protein 95. These results indicate that LF and iTBS can promote the generation of mature neurons from human iPSCs; HF may promote differentiate into glutamatergic neurons while iTBS may promote synapse formation during the differentiation.
According to the limitations of calculation of the original random early detection (RED) algorithm in linear packet loss rate. This paper proposes an improved algorithm which imposes nonlinear smooth for packet loss rate function of RED algorithm. The speed of growth of packet loss rate is relatively slow near the minimum threshold, while near the maximum threshold the speed of growth of packet loss rate is relatively faster. In this case, using the trend of the average queue length to dynamically adjust the parameters of the RED algorithm, it reduces the dependence on the parameters of the RED algorithm and enhances the stability of the algorithm. NS simulation shows that this algorithm has been significantly improved for packet loss rate, throughput and other performance.
Modal analysis, which is applied to verify the vibration characteristic of structures, is the basis of various dynamic analyses. In this paper, firstly, a cable-stayed space truss, consisting of stay cables, columns and space truss, is built in ANSYS. Secondly, modal analysis is performed to confirm the natural frequencies of the structure and 10 modes are extracted. Thirdly, a modal neutral file including all necessary information of flexible bodies is generated by ANSYS and then imported into ADAMS for further modal analysis. Finally, comparison of results between ANSYS and ADAMS are summarized and qualitative analysis is carried out aiming at the difference of natural frequencies between ANSYS and ADAMS. The simulation results indicate that the interface between the two tools has strong compatibility regardless of minor errors in it.
This paper bases on the magnetic method combining the tensor maxwell in considering magnetic saturation method, in considering magnetic saturation effect and actual switched reluctance motor stator and rotor under the premise of very wide, deduces and sets up a analytical models of radial force which can apply directly to switched reluctance motor stator and rotor with unequal extremely arc equal two circumstances analytical models of radial force. This model conforms to the actual switched reluctance motor structure and operation characteristics and structure optimization design for switched reluctance motor, electromagnetic vibration and noise prediction and control provides theory basis. With a prototype as an example, this paper calculated results and the analytical model of the finite element analysis of the results compared to verify the correctness of the analytical model built.
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