Abstract. We here report a novel biomimetic mineralization strategy for enamel remineralization by intergration of calcium phosphate loaded and thermally triggered liposomes and a self-assembly amelogenin-inspired peptide. Firstly, calcium and phosphate loaded temperature sensitive liposomes were synthesized by Interdigitation-fusion method with 1,2-bis(palmitoyl)-sn-glycero-3-phosphorcholine (DPPC) and 1,2-bis(myristoyl)-sn-glycero-3-phosphocholine (DMPC) at mass ratio of 9:1. The liposomes were stable at room temperature, but slowly released calcium and phosphate ions if heated to 37°C . Secondly, a novel polyanion amelogenin-inspired oligopeptide (Gln-Pro-Ala) 4 -Thr-Lys-Arg-Glu-Glu-Val-Asp) was synthesized by standard solid-phase. Finally, the mixture of peptide and liposomes solution was exposed to enamel surface at 37°C . The results showed oriented enamel-like hydroxylapatite evenly deposited on enamel surface.

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