Collagen-based bio-hydrogels
are undoubtedly a hot spot in the
development of biological dressings for wound healing promotion. Herein,
glutamine transaminase (TGase), a biological nontoxic cross-linker
with high specific activity and reaction rate under mild conditions,
was utilized for the self-catalytic cross-linking of the regenerated
collagen (COL) fibril hydrogel fabricated through a molecular self-assembly
method. The results showed that the natural triple helical conformation
of COL remained completely integrated after self-catalytic cross-linking
TGase, which was definitively the fundamental for maintaining its
superior bioactivity. It was worth noting that TGase could promote
the self-assembly process of COL building blocks into a higher order
D-period cross-striated structure. Also, the reconstructed TGase cross-linked
COL fibrils exhibited a higher degree of interfiber entanglements
with more straight and longer fibrils. Meanwhile, the thermal stability
of COL was significantly improved after introducing TGase. Besides,
the cytocompatibility analysis suggested that the regenerated COL
fibril hydrogel showed excellent cell growth activity and proliferation
ability when the dosage of TGase is less than 40 U/g. Further, animal
experiments indicated that the targeted COL fibril hydrogel could
significantly promote skin wound healing, exhibiting better capacity
of skin tissue for regeneration than the COL hydrogel untreated as
expected. Therefore, the reconstructed TGase cross-linked COL fibril
hydrogel could serve as a novel soft material for wound healing promotion.
Gelatin
(GE), a nontoxic and degradable biomass resource, is a
multistage hydrolysate of collagen. However, its application in adhesives
was always limited by its brittleness and poor water resistance. In
order to overcome this shortcoming, herein, epoxy-terminated hyperbranched
polymers (EHPAEs) were first selected as a cross-linking agent to
modify the GE extracted from leather solid wastes to prepare a series
of novel GE-based environmentally friendly adhesives. First, EHPAE
was synthesized and the structure of the hyperbranched polymer was
measured by Fourier-transform infrared and 1H NMR spectroscopy.
Then, the adhesives were obtained using the EHPAE to modify GE. The
adhesion effect of the prepared adhesives was evaluated by tensile
and T-peel tests on the surface of leather, in order to determine
whether this adhesive achieved the quality requirements for footwear
and bags. The result indicated that EHPAE-modified GE-based adhesives
had higher solid content, superior adhesion ability, and water resistance.
Moreover, the bonding layer of this adhesive was tough and ductile,
while the bonding layers of the recently reported GE-based adhesives
were brittle. This adhesive also surpassed the recently reported GE-based
adhesives in terms of water resistance and adhesion performance.
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