We describe a simple method for preparing Au‐TiO2/graphene (GR) nanocomposite by deposition of Au nanoparticles (NPs) on TiO2/GR substrates. The as‐prepared Au‐TiO2/GR was characterized by X‐ray diffraction (XRD) and transmission electron microscopy (TEM). The presence of Au NPs on TiO2/GR surface remarkably improves the electrocatalytic activity towards the oxidation of hydrogen peroxide (H2O2) and β‐nicotinamide adenine dinucleotide (NADH). The Au‐TiO2/GR modified glassy carbon (GC) electrode exhibits good amperometric response to H2O2 and NADH, with linear range from 10 to 200 µM and 10 to 240 µM, and detection limit of 0.7 and 0.2 µM, respectively.

The versatile role of long noncoding RNAs (lncRNAs) in plant growth and development has been established, but a systematic identification and analysis of lncRNAs in the pear has not been reported. Bud dormancy is a crucial and complicated protective mechanism for plants in winter. The roles of lncRNAs in the dormancy process remain largely unclear. In this study, we induced pear floral buds to enter into different dormant statuses by simulating four different chilling accumulation conditions. Then, a time series of RNA-seq analysis was performed and we identified 7,594 lncRNAs in Pyrus pyrifolia ‘Huanghua’ that have not been identified. The sequence and expression of the lncRNAs were confirmed by PCR analysis. In total, 6,253 lncRNAs were predicted to target protein-coding genes including 692 cis-regulated pairs (596 lncRNAs) and 13,158 trans-regulated pairs (6,181 lncRNAs). GO analysis revealed that most of lncRNAs target genes were involved in catalytic activity, metabolic processes and cellular processes. In the trend analysis, 124 long-term cold response lncRNAs and 80 short-term cold response lncRNAs were predicted. Regarding the lncRNA-miRNA regulatory networks, 59 lncRNAs were identified as potential precursors for miRNA members of 20 families, 586 lncRNAs were targets of 261 pear miRNAs and 53 lncRNAs were endogenous target mimics (eTMs) for 26 miRNAs. In addition, three cold response lncRNAs, two miRNAs and their target genes were selected for expression confirmed. The trend of their expression was consistent with the predicted relationships among them and suggesting possible roles of lncRNAs in ABA metabolic pathway. Our findings not only suggest the potential roles of lncRNAs in regulating the dormancy of pear floral buds but also provide new insights into the lncRNA-miRNA-mRNA regulatory network in plants.

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As one of the groups most affected by the epidemic, the mental health of college students during the epidemic is a focus of attention in multiple fields. Based on resource conservation theory, this study investigates the impact of COVID-19-related restrictions on college students' problematic smartphone use and mental health from two perspectives, students' individual factors and external environmental factors, and specifically explores the role of fear of missing out (FoMO), resilience and social support in this context. This study used a questionnaire method, and to control for common method bias, a multitemporal data collection strategy was used. The study used online questionnaire distribution, the final sample included 975 Chinese college students (497 males and 478 females), and of these, 10.3% were freshmen, 31.9% were sophomores, 31.6% were juniors, 12.3% were seniors, and 13.9% were postgraduates. The results of this study showed the following: (1) Perceived COVID-19-related strain was positively correlated with perceived FoMO, problematic smartphone use and mental health problems (depression, anxiety, stress) among college students. (2) FoMO partially mediated the relationship between perceived COVID-19-related restrictions and problematic smartphone use, and it fully mediated the relationship between perceived COVID-19-related restrictions and mental health problems. (3) Resilience and social support co-moderated the relationship between FoMO and problematic smartphone use or mental health problems (depression, anxiety, stress).

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