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This study successfully fabricated BPA-imprinted poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) (poly(4-VP-co-EGDMA)) quartz crystal microbalance (MIP-QCM) sensors on a silica skeleton surface and gold pinholes of silica inverse opal through surface-initiated atom transfer radical polymerization (SI-ATRP). The sensing features of the two MIP films on the structured silica surface and nano-scale local gold surface were investigated by measuring the resonant frequency change (∆f) in QCM sensors. The ∆f values for the p-MIP (MIP on gold pinholes) and s-MIP films (MIP on silica skeleton surface) were obtained with the ∆f value of −199 ± 4.9 Hz and −376 ± 19.1 Hz, respectively, whereas for p-/s-NIP films, the ∆f values were observed to be −115 ± 19.2 Hz and −174 ± 5.8 Hz by the influence of non-specific adsorption on the surface of the films. Additionally, the imprinting factor (IF) appeared to be 1.72 for p-MIP film and 2.15 for s-MIP film, and the limits of quantitation (LOQ) and detection (LOD) were 54.924 and 18.125 nM (p-MIP film) and 38.419 and 12.678 nM (s-MIP film), respectively. Using the Freundlich isotherm model, the binding affinity of the BPA-imprinted films was evaluated. This was measured in an aqueous solution of BPA whose concentration ranged between 45 and 225 nM. It was found that the p-MIP film (m = 0.39) was relatively more heterogeneous than the s-MIP film (m = 0.33), both of which were obtained from the slope of the linear regressions. Finally, the selectivity of the MIP-QCM sensors for BPA detection was determined by measuring the effect of other analogous chemicals, such as bisphenol F (BPF), bisphenol AP (BPAP), and bisphenol B (BPB), in aqueous solutions. The selectivity coefficients (k*) of the two MIP films had ~1.9 for the p-MIP and ~2.3 for the s-MIP films, respectively. The results reveal that, with respect to signal amplification of the QCM sensors, the s-MIP film has better sensing features and faster detection responses than the p-MIP film.

A visual observation of the bending angle changes of molecularly imprinted hydrogel strips with a one-sided porous pattern for the novel and easy detection of proteins.

In this study, a chalcone-branched polyimide (CB-PI) was synthesized by the Steglich esterification reaction for selective recognition of the toxic peptide melittin (MEL). MEL was immobilized on a nanopatterned poly(dimethylsiloxane) (PDMS) mold using a conventional surface modification technique to increase binding sites. A stripe-nanopatterned thin CB-PI film was formed on a quartz crystal (QC) substrate by simultaneously performing microcontact printing and ultraviolet (UV) light dimerization using a MEL-immobilized mold. The surface morphology changes and dimensions of the molecularly imprinted polymer (MIP) films with stripe nanopatterns (S-MIP) were analyzed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The sensing signals (Δf and Q e ) of the S-MIP sensor were investigated upon adsorption in a 100-μL dilute plasma solution containing 30 μg/ mL MEL, and its reproducibility, reuse, stability, and durability were investigated. The S-MIP sensor showed high sensitivity (5.49 mL/mg) and coefficient of determination (R 2 = 0.999), and the detection limit (LOD) and the quantification limit (LOQ) were determined as 0.3 and 1.1 μg/mL, respectively. In addition, the selectivity coefficients (k*) calculated from the selectivity tests were 2.7−5.7, 2.1−4.3, and 2.8− 4.6 for bovine serum albumin (BSA), immunoglobulin G (IgG), and apamin (APA), respectively. Our results indicate that the nanopatterned MIP sensors based on CB-PI demonstrate great potential as a sensing tool for the quantitative analysis of biomolecules.

Surface imprinting used for protein recognition in functional cavities is highly effective in imprinting biomacromolecules to avoid template encapsulation during the formation of a molecularly imprinted polymer (MIP) matrix. Herein, we introduce a facile surface-imprinting method based on two-step spin-coating and photopolymerization to design highly efficient imprinted sites on polymeric films to detect trypsin (TRY). Well-distributed template imprinting is successfully achieved for maximized sensing responses by controlling the composition of functional monomers and crosslinkers in the precursor solution and the concentration of TRY in the imprinting solution. The MIP film exhibits higher sensitivity (−841 ± 65 Hz/(μg/mL)) with a coefficient of determination of 0.970 and a higher imprinting factor of 4.5 in a 0.24 µg/mL TRY solution compared to the nonimprinted polymer (NIP) film. Moreover, the limit of detection and limit of quantification are calculated to be 25.33 and 84.42 ng/mL, respectively. Finally, the selectivity coefficient is within the range of 3.90–6.78 for TRY against other proteins. These sensing properties are superior to those of the corresponding nonimprinted polymer matrix. Thus, the proposed facile surface-imprinting method is highly effective for protein imprinting with high sensitivity and selectivity.