Background: Phosphorus (P) is essential for plant growth and development, and low-phosphorus (LP) stress is a major factor limiting the growth and yield of soybean. Recently, long noncoding RNAs (lncRNAs) have been reported to be key regulators in response to stress conditions in plants. In soybean, however, how LP stress mediates biogenesis of lncRNAs remains unclear. Results: In this study, to explore the response mechanisms of lncRNAs to LP stress, we used the roots of two representative soybean genotypes with opposite P deficiency responsiveness, a P-sensitive genotype (Bogao) and a P-tolerant genotype (NN94156), to construct RNA sequencing (RNA-seq) libraries. In total, 4,166 novel lncRNAs including 525 differently expressed (DE) lncRNAs were identified across two genotypes at different P levels. GO and KEGG analyses indicated that numerous DE lncRNAs might be involved in diverse biological processes of phosphate, such as lipid metabolic process, catalytic activity, cell membrane formation, signal transduction, nitrogen fixation. Moreover, lncRNA-mRNA-miRNA and lncRNA-mRNA networks were constructed and several promising lncRNAs were identified, which may have highly valuable for further analysis the mechanism in response to LP stress in soybean.Conclusions: These results revealed that LP stress can significantly alter the genome-wide profiles of lncRNAs, especially for P sensitive genotype Bogao. Our findings increase the understanding and provides new insights into the function of lncNRAs responses to P stress in soybean.

Background: Phosphorus (P) is essential for plant growth and development, and low-phosphorus (LP) stress is a major factor limiting the growth and yield of soybean. Long noncoding RNAs (lncRNAs) have recently been reported to be key regulators in the responses of plants to stress conditions, but the mechanism through which LP stress mediates the biogenesis of lncRNAs in soybean remains unclear.Results: In this study, to explore the response mechanisms of lncRNAs to LP stress, we used the roots of two representative soybean genotypes that present opposite responses to P deficiency, namely, a P-sensitive genotype (Bogao) and a P-tolerant genotype (NN94156), for the construction of RNA sequencing (RNA-seq) libraries. In total, 4,166 novel lncRNAs, including 525 differentially expressed (DE) lncRNAs, were identified from the two genotypes at different P levels. GO and KEGG analyses indicated that numerous DE lncRNAs might be involved in diverse biological processes related to phosphate, such as lipid metabolic processes, catalytic activity, cell membrane formation, signal transduction, and nitrogen fixation. Moreover, lncRNA-mRNA-miRNA and lncRNA-mRNA networks were constructed, and the results identified several promising lncRNAs that might be highly valuable for further analysis of the mechanism underlying the response of soybean to LP stress.Conclusions: These results revealed that LP stress can significantly alter the genome-wide profiles of lncRNAs, particularly those of the P-sensitive genotype Bogao. Our findings increase the understanding of and provide new insights into the function of lncRNAs in the responses of soybean to P stress.

The preparation of hydrogen by photocatalytic principle is an important subject in the future energy direction. This article intends to design the catalytic structure of a crystalline (amorphous) TiO2/two-dimensional MoS2 sandwich structure supporting a single-atom, and explore the effect of this special structure on the photocatalytic reaction.Calculating the effective mass, electrostatic potential, light absorption spectrum and other related properties by DFT method, the analysis shows that the sandwich structure catalyst has good carrier transfer ability and electron-hole separation ability. Except for the Pt@Amorphousr-TiO2/MoS2 sandwich structure single-atom catalyst, which only has an excellent effect on the oxygen evolution of water, the rest of the interlayer catalysts have the two characteristics of photocatalytic hydrogen evolution and oxygen evolution.The new catalyst designed in this paper has innovative design ideas and high-efficiency research and development value.