Objective Mechanical factors play a critical role in the physiology and pathology of articular cartilage, although the mechanisms of mechanical signal transduction are not fully understood. We examined the hypothesis that type VI collagen is necessary for mechanotransduction in articular cartilage, by determining the effects of type VI collagen knockout on the activation of the mechano-osmosensitive calcium-permeable channel, transient receptor potential vanilloid 4 (TRPV4), osmotically-induced chondrocyte swelling, and pericellular matrix (PCM) mechanical properties. Methods Confocal laser scanning microscopy was used to image TRPV4-mediated calcium signaling and osmotically-induced cell swelling in intact femora from 2 and 9 month old wild type (WT) and type VI collagen deficient (Col6a1−/−) mice. Immunofluorescence-guided atomic force microscopy was used to map PCM mechanical properties based on the presence of perlecan. Results Hypo-osmotic stress induced TRPV4-mediated calcium signaling was increased in Col6a1−/− mice relative to WT controls at 2 months. Col6a1−/− mice exhibited significantly increased osmotically-induced cell swelling and decreased PCM moduli relative to WT controls at both ages. Conclusion In contrast to our original hypothesis, type VI collagen was not required for TRPV4-mediated Ca2+ signaling; however, knockout of type VI collagen altered the mechanical properties of the PCM, which in turn increased the extent of cell swelling and osmotically-induced TRPV4 signaling in an age-dependent manner. These findings emphasize the role of the PCM as a transducer of mechanical and physicochemical signals, and suggest that alterations in PCM properties, as may occur with aging or osteoarthritis, can influence mechanotransduction via TRPV4 or other ion channels.

Objective: This study aims to investigate the differences in public vaccination preference for the COVID-19 vaccine with different personality characteristics. Methods: Based on the Big Five Personality Inventory (BFI-10), a total of 1200 respondents were categorized by personality characteristics using Latent Profile Analysis (LPA). The preference of members the public with different personality characteristics for COVID-19 vaccination was investigated based on a discrete choice experiment (DCE). Results: All respondents were divided into three groups, named the General and Stable type (79.67%), Conscientious and Agreeable type (9.5%), and Open and Extroverted type (10.83%). For the percentage importance of vaccine attributes, both the General and Stable type and Conscientious and Agreeable type respondents considered cost to be the most important (41.93% and 34.95% respectively). However, the Open and Extroverted type respondents considered efficacy as the most important (31.05%). In our conditional logit model (CLOGIT), for vaccine adverse effects, the General and Stable type and Conscientious and Agreeable type respondents preferred “very mild”, while the Open and Extroverted type preferred “mild” (OR:1.108, 95%CI 0.977–1.256). The Open and Extroverted type had a higher willingness to pay (WTP) for the most preferred vaccine level compared to the other types. Conclusions: The Open and Extroverted respondents have the highest willingness to vaccinate. The General and Stable type and Conscientious and Agreeable respondents think that the cost of the vaccine is the most important attribute, and prefer the mildest side effects. The Open and Extroverted type think that vaccine efficacy is the most important attribute, prefer “mild” side effects, and have higher willingness to pay for their favorite vaccine level.

Entrepreneurship plays a significant role in promoting the social and economic development of a country. At present, entrepreneurship education is widely carried out in universities and colleges in order to improve students’ entrepreneurial ability, and then to provide support for the formation of a comprehensive entrepreneurial situation. As entrepreneurship education has gradually become a hot topic of teaching for innovation and entrepreneurship education of international students, studies on the influencing mechanism of entrepreneurship education of international students in relation to their entrepreneurial behavior are conducive to providing theoretical basis and empirical evidence for international students’ entrepreneurship education, so as to pertinently guide international students’ innovation and entrepreneurship practice. This study aims to explore the influence of entrepreneurship education, government support for entrepreneurship, global competence, entrepreneurial self-efficacy, and entrepreneurial intention on the entrepreneurial behavior of international students in the Republic of Korea (“Korea”). It summarized and drew on the results of the existing literature research. According to the contents and points of research, this study takes the international students studying in Korea as the sample and uses statistical analysis software, SPSS 22.0 and AMOS 24.0, to establish a structural equation model to conduct empirical study on the influencing mechanism of entrepreneurial behavior of international students in Korea, so as to better understand the influence of entrepreneurship education in Korean universities and colleges on entrepreneurial behavior of international students in Korea. Based on the analysis results, this study puts forward the theoretical basis for the policies related to effective management of entrepreneurship, which will help alleviate the unemployment of young people studying in Korea and the tight labor market supply and demand.

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The uncertainty, immediate hazards, severe consequences and broad impact of medical emergencies are far beyond the scope of ordinary medical treatment, calling for special attention. The importance of constructing hospital emergency management system has been increasingly reflected. The system not only needs the practical solution of hospital management at the present stage, but also the working direction of the hospital management in the future. In this paper, the emergency satisfaction survey was conducted on the patients in two first-class tertiary hospitals in Shanghai. The service satisfaction scores of a hospital with integrated traditional Chinese medicine (TCM) and Western medicine as well as a general hospital were [Formula: see text] and [Formula: see text], respectively. There was a significant difference in the service satisfaction scores between the two hospitals ([Formula: see text]). Poor network signal was a vulnerability problem in the dimension of medical auxiliary facilities in both hospitals, accounting for the highest proportion. For the dimension of medical building, the main vulnerability problems were wall cracking and uneven ground, accounting for a high proportion. In the dimension of medical process, the parking inconvenience of a hospital with integrated traditional Chinese and Western medicine was particularly serious, accounting for 81.7%. In the dimension of medical service, the vulnerability of “medical staff overwork” in a general hospital was more prominent, accounting for 39.10%, which was significantly higher than that in a hospital with integrated TCM and Western medicine. In terms of service attitude, the vulnerability of the two hospitals was represented as the poor service attitude of doctors, nurses and managers. In the medical safety dimension of the two hospitals, “lack of assistance in emergencies” and “hospital equipment failures” were the main vulnerability issues. From the perspective of patients, this paper analyzed the current vulnerability problems of the hospital, identified the actual and potential risks of hospital emergency management and put forward suggestions on adjusting the spatial layout, strengthening internal management and comprehensively improving employees’ emergency awareness and skills. This will provide a reference for the hospital’s emergency management system to make necessary adjustments to meet the practical needs.