Various nanostructures, including liposomes, dendrimers, polymers, carbon or silicon materials, and metal nanoparticles seems to be evaluated as carriers in different gene delivery systems. Muscle cells are target of modern gene medicine, due to of the much pathology (arrhythmias, channels pathological mutation, abnormal apoptosis) with limited curability by traditional drugs. The zeta potential of the muscle cells were identified by non-traditional method of DLS. This was starting point to setting of parameters for experimental liposome, the liposome surface charge have been tested and modified by cationic plasmid and final complex was evaluated as delivery system of DNA into the muscle cardiac cells.

This paper describes the design of equipment for measuring and temperature control solutions with an accuracy of temperature measurement to ± 0.2 °C from 25 °C to 45 °C. It describes the basic blocks of the proposed facility and the results obtained in testing the liquid volume to 2 litre. The proposed facility is designed as an autonomous system, but allows setting and displaying the control on your compute. The device implements two algorithms and autonomic control of the PSD and the tri-state control. Designed and implemented system for temperature control solutions is fully operational and immediately applicable to real requirements in practice.

Monophasic action potential (MAP) can be recorded from the heart surface by optical method via fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which a new ratiometric recording setup is used for recording MAPs from isolated rabbit hearts perfused according to Langendorff. MAPs recorded by new ratiometric recording setup are compared with simple optical MAP measurement without ratiometry.

Biological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.

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