This report is concerned with the macroscopic distribution of the conduction system in the ventricles of the beef heart as revealed by multiple injections and subsequent dissection of more than fifty specimens.Hearts were obtained directly from the slaughterhouse and the residual blood was immediately washed out by passing a stream of tap-water into the coronary arteries. The specimens were then placed in an ice-box for forty-eight hours to permit rigor mortis to pass off, this preliminary step being of considerable value in facilitating the spread of the injection material.The following procedure was adopted for exposure of the inner ventricular surfaces with minimal injury to the conduction system. To open the left ventricle, first slit the wall of the left auricle posteriorly as far as the auriculoventricular groove, and then cut through the ventricular wall from base to apex in a plane midway between the two papillary muscles.As there are no obvious external landmarks, it is well to pass a finger into the ventricular cavity between the two papillae and use it as a guide. Slit the aorta so as to pass between the posterior and left cusps1 of the semilunar valve, and continue the cut through the wall of the left auricle and anterior cusp of the mitral valve. Finally, transect all chordae tendineae. To open the right ventricle, first slit The B.K.A. has been followed in the nonienclature of the valve cusps.
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