Six glycerolphosphate-containing tetraheteroglycans, a, b-1, b-2, b-3, b-4, and b-5, have been purified from the formamide extracts of Streptococcus sanguis by alcohol and acetone precipitations, Sephadex G-75, and diethylaminoethyl-cellulose column chromatography. The polysaccharides were judged as at least 95% pure by analytical disc gel electrophoresis and immune double diffusion against rabbit antiserum. They were shown to be cell wall polysaccharides, since they formed a single band of identity in immune double diffusion with partially purified polysaccharide extracted from a purified cell wall preparation of S. sanguis. The polysaccharides were composed of L-rhamnose, I-glucose, ani N-acetyl D-glucosamine in a similar molar ratio, but varied in their glycerol and phosphate contents. They exhibited four different mobilities in polyacrylamide disc gel electrophoresis at pH 8.9. When they were treated with formamide at 170 C for 20 min, the faster moving polysaccharide(s) yielded polysaccharides with mobilities corresponding to the other slower moving polysaccharides. These results indicate that the polysaccharides originated from the same cell wall polysaccharide and were produced as a result of breakage in the phosphodiester bonds during the formamide extraction procedure. A preliminary structural study shows that the terminal reducing sugar is L-rhamnose and that the glycerol moiety is probably linked to the polysaccharide through a phosphodiester bond.Glycerolphosphate-containing polymers have been found in many streptococcal cell envelopes in the form of teichoic acids (9,22,23, 28). Heymann et al. (14) have also reported the presence of glycerolphosphate in a cell wall polysaccharide-peptidoglycan complex isolated from group A streptococcal cell walls. The glycerolphosphate was shown to be involved in the linkage between the reducing terminal of C-polysaccharide and the N-acetylmuramic acid moiety of peptidoglycan.Most recently we have isolated a glycerolphosphate-containing cell wall polysaccharide from Streptococcus sanguis; however, our results showed that glycerolphosphate is not linked to the reducing terminal L-rhamnose by a glycosidic bond as was reported by Heymann et al. (14) for Streptococcus pyogenes.

A simple reversed-phase HPLC method was developed for routine analysis of lactic acid in Ciprofloxacin Injection. The analysis involves simple sample preparation to ensure complete hydrolysis of the oligomers of lactic acid. Using these conditions, recoveries ranging from 97.5 to 99.9% at three levels of the label claim were obtained. The standard solution and the sample solution were shown to be stable for at least 41 hours when stored at room temperature. Tests done have also demonstrated that the method is stable despite small changes in experimental conditions. Data supporting the development and validation of this method are presented.