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Adipose tissues and other tissues of the pig have been examined for the presence of the mitochondrial "uncoupling protein," characteristic of brown adipose tissue, in order to assess whether brown fat is present in this species. Mitochondria were prepared from various tissues and the proteins separated on the basis of molecular weight by sodium dodecyl sulphate--polyacrylamide gel electrophoresis. Immunoblotting procedures were then used to probe for uncoupling protein, employing a rabbit anti-(rat uncoupling protein) serum. Pigs were examined at 4 days, 4 weeks, and 8 weeks of age. No evidence for the presence of uncoupling protein was found at any of these ages. The protein was, however, readily detected in brown adipose tissue from rats, mice, golden hamsters, guinea pigs, Richardson's ground squirrel, and lambs. An additional group of pigs was acclimated to the cold (10 degrees C) for a period of 10 days prior to the examination of tissues, but again uncoupling protein was not detected in any tissue. These results indicate that uncoupling protein is either absent from adipose tissues of the pig or is present at such a low concentration that it is unlikely to support thermogenesis. It is concluded that the pig does not contain adipose tissue that is functionally "brown;" adipose tissues in this species appear to be exclusively "white."

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The study was commenced a t i n s t i t u t i o n of a s s i s t e d v e n t i l at i o n. Most common beginning s e t t i n g s included PEEP 4 , PIP 25, 5'102 100%. One hour l a t e r , stepwise changes were made i n PEEP/ PIP t o produce increments of 1-2 cm H20 Paw pressure. Increases were made t o achieve maximum s e t t i n g s of PEEP 12 and Paw 22. Res u l t i n g ICP varied only from 4-9 cm H20. No values approached the 18-20 cm Hz0 value which i s considered abnormal. Blood oressure did not change durine the studv.