Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is a glucosyltransferase that catalyzes synthesis of polyphenolic into polyphenolic glycosides. The synthesis of glycosides via transglycosylation reaction of Trichoderma viride CGTase was studied in the presence of polyphenolic constituents from Moringa oleifera extract. The transglycosylation products were determined through 1 H and 13 C-NMR to be gallic acid-4-O-b-glucopyranoside (GAGP), ellagic acid-4-O-b-glucopyranoside (EAGP) and catechin-4 0 -Oglucopyranoside (CGP), respectively. The EAGP was furthermore subjected on its antioxidant activities.Results showed that IC 50 value of EAGP against 1-diphenyl-2-picryl hydrazyl was higher (46.12 mg/mL) than ascorbic acid (35.96 mg/mL), butylated hydroxytoluene (BHT, 39.73 mg/mL), and a-tocopherol (42.62 mg/mL), respectively. The IC 50 of EAGP against 2,2 0 -azinobis-3-ethylbenzothiazoline-6-sulfonic acid was found to be 64.01 mg/mL as compared to ascorbic acid (30.13 mg/mL), BHT (38.09 mg/mL), and a-tocopherol (54.84 mg/mL), respectively. The IC 50 value of EAGP against H 2 O 2 was found to be 51.90 mg/mL, while for BHT and ascorbic acid were 44.18 and 47.18 mg/mL, respectively. Moreover, the IC 50 on metal chelating ability of EAGP was found to be 45.42 mg/mL as compared to ethylene diamine tetra acetic acid was 28.24 mg/mL. Practical applicationsThis paper reports on technology to synthesis and stabilize polyphenolic constituent that was extracted from leaves of Moringa oleifera as source of bioactive compounds by application of transglycosylation reaction cyclodextrin glucanotransferase derived from fungal culture of Trichoderma viride. The enzymatically synthesized products could be qualitatively analyzed using thin layer chromatography as well as quantitatively estimated using high-performance liquid chromatography and were furthermore determined as transfer products those are referred to arbutin as authentic standard for polyphenol glycoside. The transfer product has been successfully identified as new novel structures of polyphenol glycosides according to their respective characteristics that could be recognized through NMR spectroscopy as constituents of GAGP, EAGP, and CGP, respectively.The EAGP as enzymatic transglycosylation reaction product was furthermore assayed through in vitro analysis regarding with its functional properties, especially antioxidant activity. K E Y W O R D S antioxidant, polyphenol glycoside, transglycosylation, Trichoderma viridae

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