The aim of this study was: (i) understand how upstream signaling modulated TOR-SnRK1 nexus; and (ii) establish an interplay between SnRK1-TOR nexus, sugar availability, sucrolytic enzyme activities, expression level of key genes related to signalling and sugar metabolism, including trehalose, in in vitro-grown of ‘Myrobalan 29C’plum rootstock (Prunus cerasifera). Explants were cultivated in Murashigue and Skoog medium (MS) with trehalose (0; 1,0 and 10 mM). In 3 days, the antagonistic role of PcSnRK1 and PcTOR was confirmed in plants treated with 10mM trehalose, possibly indicating that ‘Myrobalan 29C’ was not in a stress condition. Furthermore, a PcTREA up-regulation was observed, which can lead to glucose accumulation, that in turn is precursor of sorbitol synthesis. Regarding the growth parameters evaluated after 21 days of in vitro culture, the uppermust number of shoots and explant length was observed at 10mM trehalose. Such positive response may be due to an increase in Glucose and UDP-Glc content, direct products of sucrose synthase (SuSy) enzyme. Consistent with these results, the highest availability of these molecules may be the upstream signal for TOR-activation. Interestingly, in this same condition, a sucrose accumulation was observed, which may also have contributed to PcTOR up-regulation and ameliorate in growth parameters.

No abstract

Necrophagous flies are of great importance for human and animal health. In places where their development occurs, parasitoids can be used as a tool to control these dipterans. In Brazil, the fauna of these parasitoids has been investigated in some regions. However, in Rio Grande do Sul, it is known the occurrence of only one species. Thus, this study aimed to create the first list of parasitoids in flies of medical and veterinary importance in Southern Brazil. Collections took place in the municipality of Pelotas (31º 42’ S; 52º 18’ W). Three replicates consisting of a 500 g bovine liver chunk placed in a tray were exposed to open air for 20 days. Then pupae were individualized and observed until their emergence. We identified 4,882 adult flies of Calliphoridae, Fanniidae, Muscidae, and Sarcophagidae. 4,040 parasitoids emerged, belonging to eight species, of which Nasonia vitripennis, Spalangia cameroni, Spalangia chontalensis, and Tachinephagus zealandicus are new records in Rio Grande do Sul state. Also, three undescribed species of the genus Aphaereta were collected. In spite of being the first attempt to inventory the fauna of hymenopteran parasitoids, this study may help in the development of management programs of these dipterans in the region.