Diatoms possess an impressive capacity for rapidly inducible thermal dissipation of excess absorbed energy (qE), provided by the xanthophyll diatoxanthin and Lhcx proteins. By knocking out the Lhcx1 and Lhcx2 genes individually in Phaeodactylum tricornutum strain 4 and complementing the knockout lines with different Lhcx proteins, multiple mutants with varying qE capacities are obtained, ranging from zero to high values. We demonstrate that qE is entirely dependent on the concerted action of diatoxanthin and Lhcx proteins, with Lhcx1, Lhcx2 and Lhcx3 having similar functions. Moreover, we establish a clear link between Lhcx1/2/3 mediated inducible thermal energy dissipation and a reduction in the functional absorption cross-section of photosystem II. This regulation of the functional absorption cross-section can be tuned by altered Lhcx protein expression in response to environmental conditions. Our results provide a holistic understanding of the rapidly inducible thermal energy dissipation process and its mechanistic implications in diatoms.

The West Antarctic Peninsula (WAP) is a highly productive polar ecosystem where phytoplankton dynamics are regulated by intense bottom-up control from light and iron availability. Rapid climate change along the WAP is driving shifts in the mixed layer depth and iron availability. Elucidating the relative role of each of these controls and their interactions is crucial for understanding of how primary productivity will change in coming decades. Using a combination of ultra-high-resolution variable chlorophyll fluorescence together with fluorescence lifetime analyses on the 2017 Palmer Long Term Ecological Research cruise, we mapped the temporal and spatial variability in phytoplankton photophysiology across the WAP. Highest photosynthetic energy conversion efficiencies and lowest fluorescence quantum yields were observed in iron replete coastal regions. Photosynthetic energy conversion efficiencies decreased by~60% with a proportional increase in quantum yields of thermal dissipation and fluorescence on the outer continental shelf and slope. The combined analysis of variable fluorescence and lifetimes revealed that, in addition to the decrease in the fraction of inactive reaction centers, up to 20% of light harvesting chlorophyll-protein antenna complexes were energetically uncoupled from photosystem II reaction centers in iron-limited phytoplankton. These biophysical signatures strongly suggest severe iron limitation of photosynthesis in the surface waters along the continental slope of the WAP.

Optical water classification based on remote sensing reflectance (Rrs(λ)) data can provide insight into water components driving optical variability and inform the development and application of bio-optical algorithms in complex aquatic systems. In this study, we use an in situ dataset consisting of hyperspectral Rrs(λ) and other biogeochemical and optical parameters collected over nearly five years across a heavily urbanized estuary, the Long Island Sound (LIS), east of New York City, USA, to optically classify LIS waters based on Rrs(λ) spectral shape. We investigate the similarities and differences of discrete groupings (k-means clustering) and continuous spectral indexing using the Apparent Visible Wavelength (AVW) in relation to system biogeochemistry and water properties. Our Rrs(λ) dataset in LIS was best described by three spectral clusters, the first two accounting for the majority (89%) of Rrs(λ) observations and primarily driven by phytoplankton dynamics, with the third confined to measurements in river and river plume waters. We found AVW effective at tracking subtle changes in Rrs(λ) spectral shape and fine-scale water quality features along river-to-ocean gradients. The recently developed Quality Water Index Polynomial (QWIP) was applied to evaluate three different atmospheric correction approaches for satellite-derived Rrs(λ) from the Sentinel-3 Ocean and Land Colour Instrument (OLCI) sensor in LIS, finding Polymer to be the preferred approach. Our results suggest that integrative, continuous indices such as AVW can be effective indicators to assess nearshore biogeochemical variability and evaluate the quality of both in situ and satellite bio-optical datasets, as needed for improved ecosystem and water resource management in LIS and similar regions.

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In the Equatorial Atlantic nitrogen availability is assumed to control phytoplankton dynamics. However, in situ measurements of phytoplankton physiology and productivity are surprisingly sparse in comparison with the North Atlantic. In addition to the formation of the Equatorial cold tongue in the boreal summer, tropical instability waves (TIWs) and related short-term processes may locally cause episodic events of enhanced nutrient supply to the euphotic layer. Here, we assess changes in phytoplankton photophysiology in response to such episodic events as well as short-term nutrient addition experiments using a pair of custom-built fluorometers that measure chlorophyll a (Chl a) variable fluorescence and fluorescence lifetimes. The fluorometers were deployed during a transatlantic cruise along the Equator in the fall of 2019. We hypothesized that the Equatorial Atlantic is nitrogen-limited, with an increasing degree of limitation to the west where the cold tongue is not prominent, and that infrequent nitrate injection by TIW related processes are the primary source alleviating this limitation. We further hypothesized phytoplankton are well acclimated to the low levels of nitrogen, and once nitrogen is supplied, they can rapidly utilize it to stimulate growth and productivity. Across three TIW events encountered, we observed increased productivity and chlorophyll a concentration concurrent with a decreased photochemical conversion efficiency and overall photophysiological competency. Moreover, the observed decrease in photosynthetic turnover rates toward the western section suggested a 70% decrease in growth rates compared to their maximum values under nutrient-replete conditions. This decrease aligned with the increased growth rates observed following 24 h incubation with added nitrate in the western section. These results support our hypotheses that nitrogen is the limiting factor in the region and that phytoplankton are in a state of balanced growth, waiting to “body surf” waves of nutrients which fuel growth and productivity.