Objectives: To investigate the up-regulation of chemokine ligand 20 (CCL20) in chronic rhinosinusitis mucosa and to localize the distribution of CCL20 in the human paranasal sinus mucosa.
By screening Caenorhabditis elegans cDNA libraries, overlapping cDNA clones encoding DNA topoisomerase I were obtained. An open reading frame of 751 amino acids was found in the 3.2-kb cDNA sequence. The open reading frame has 54% and 50% identities to the amino acid sequences of human and Drosophila melanogaster DNA topoisomerases I, respectively. Northern blot analysis showed the presence of an mRNA of 3.4 kb which suggests that the cDNA sequence is close to full length. The 72-kDa C-terminal polypeptide expressed in Escherichia coli cells showed catalytic DNA topoisomerase I activity. The DNA topoisomerase I gene was mapped to position 18 of chromosome I by screening polytene YAC plasmid DNAs.
Summary
Watermarking is a mechanism in which owner of the audio file hides the watermark information into a audio for various applications. The identity of the owner of the audio file is hidden in the audio, which is known as watermark. In this article, a quantization‐based audio watermarking using singular value decomposition (SVD) is proposed. The original audio signal is converted into non overlapping two dimensional matrix blocks. The SVD is applied to each block. The watermark is embedded into audio signal by quantization of largest singular value of the block. The watermark is extracted blindly without using original audio signal. Experimental results show the watermark's high imperceptibility in the audio signal and good performance against Stirmark as well as traditional signal processing attacks. Compared with other audio watermarking methods, our method has higher embedding capacity and robust against various traditional signal processing attacks.
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