We present a procedure for designing to control the three-dimensional light-intensity distribution near focus. Our method is based on the use of a series of figures of merit that are properly defined to describe the effect of general complex pupil functions. As a practical implementation, we have applied our method to obtain super resolving continuous smoothly varying phase-only filters. The advantages of these kinds of filters are that they do not produce energy absorption and they are easy to build with a phase-controlling device such as a deformable mirror. Results of comparisons between the performance of our method and that of other phase-filter designs are provided.

High resolution wavefront sensors are devices with a great practical interest since they are becoming a key part in an increasing number of applications like extreme Adaptive Optics. We describe the optical differentiation wavefront sensor, consisting of an amplitude mask placed at the intermediate focal plane of a 4-f setup. This sensor offers the advantages of high resolution and adjustable dynamic range. Furthermore, it can work with polychromatic light sources. In this paper we show that, even in adverse low-light-level conditions, its SNR compares quite well to that corresponding to the Hartmann-Shack sensor.

Sexual investment in P. odoratum has shown a marked variability within plants, among plants, and between populations, which confirms the importance of analysing sex expression in plants of this type. Differences in relative investment in male and female components (phenotypic gender) are reflected in the functional gender and it would be expected that the evolution of sexual specialization in Polygonatum odoratum would be promoted.

A novel procedure to design axial and transverse superresolving pupil filters for the 4Pi-confocal microscope is presented. The method is based on the use of a series of figures of merit developed to describe the effect of inserting two identical filters in the two arms of the illumination path of the microscope. As a practical implementation, we have applied our method to obtain superresolving continuous phase-only filters. Different resolution-improving phase functions are shown for the transverse and the axial direction. These filters provided axial gain up to 1.3 and transverse gain up to 1.4 without an increase in sidelobes.

The ophthalmic applications of a diffractive trifocal lens design with adjustable add powers and light distribution in the foci are investigated. Axial PSFs of the trifocal lenses are calculated and analyzed as a function of the design parameters and the eye pupil size. The optical performance in actual eyes is also simulated by including the measured ocular wave aberration functions of human eyes in the calculation of transverse and axial PSFs, and Strehl ratio axial variation. The effect of the polychromatic character of natural light has also been considered. The calculus and simulation method of this paper can be applied for the design and analysis of any other kind of diffractive or refractive multifocal contact or intraocular lens.