Ite('eived for publication Jttne 1, 1959 StU(ly of the enzymes of skin an(l l)articuiarlY of el)i(lerlflis has i)eeli llan(licappe(l 1)y its histological complexity. The micro methods devised by I)r. 0. H. Lowry have pernlitte(l accurate Samfling of various (('11 ty)es in brain, an(l other structures. In this a jer, (lata are l)resentecl testing :tIuI vali(lating nlinor nlodiflcations of these lilethO(15 that are useful for the studs' of various ('elI types in litlIllali skin. Study of the enzynles of skin :tiicl especially of ('j)i(lerIllis has 1)een Ilalldicapped also by iifficulty in se)arating and honiogenizing elji(lerl1lis, an(I at't'urate sanl)ling of the at'tive layers an(l structures. At'curate sanl)hng is necessary 1)ecaUSe the sul)jacent (lernus is relatively inactive. Soine of tllese difficulties have l)een circulllveflte(l so that sn all 5itIlll)les of particular tell tVl)es (all 1)e analvze(l. 'lilt' methods are sufficiently l)reeiSe and setisitive to l)('rlllit conll)arison of nlicrogr:ull 5aI1 l)les. 'i'his)a)er rel)orts nleasurenlent of five emizvines fromii different 11letal)Olic l)atll\\'aYs in 5-20 'y sahlil)les (wet weight) of honlogellates of hunlan ('l)i(lerlllis, or 0.5 to 5 y sanll)les fronl freeze (lried sections. ihe activity of four of these enzvnu's (It) not a i ear to llave 1)eel1 reported in ('l)i(lerluis I)efOi(. I)ata slll)wing the distril)UtiOn of these enzvnles in el)i(lernlis 1(11(1 (lernlis of the sole of the foot and skill of the groin and mdivi(lual samples of hair follicles, sel)aceous aIl(l sweat glands are presented. These may serve as baseline observations for studies for carcinogenesis, healing wounds and other physiological or)atllOl0gical l)ro('esses. Ml:TIlol)s To Ol)taih! tile ol)timt!m cOn(lit ions for the metho(1s human epidermal homogenates were used. The epidermis was separated by our modifi (at it)!! (2) of t lie ''stretch met hod'' of \an Scott From the I)epart

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