Phosphopyridine Nucleotide Linked Dehydrogenases, Aldolase and Fumarase in Mouse Breast

Hershey, Falls B.; Lewis, Charles; Johnston, Gloria; Murphy, Josephine

doi:10.1177/11.2.224

Cited by 8 publications

(2 citation statements)

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“…The material included in the present study was stored mostly for only a few weeks. FH was found to behave similarly to ICDH (NAD), as reported earlier by Hershey et al [18]. It was found that there was a significant linear regression of activity on storage time.…”

Section: Selection El Neurodermite Materials For Icdh (Nad)supporting

confidence: 80%

NAD and NADP dependent isocitrate dehydrogenase and fumarate hydratase activities in normal human skin and in some maculosquamous diseases of the skin

Hammar

1973

Arch. Derm. Res.

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Summary. Activities of the isocitrate dehydrogenases, ICDH(NAD) andICDH (NADP), and of fumarate hydratase, FH, were measured in subcorneal and basal epidermal layers in patients with psoriasis, neurodermatitis, lichen planus and pityriasis rosea and in healthy controls. Lowry's microtechniques were used. Since NADH formed by the ICDH reaction required high analytical sensitivity, this dinucleotide was measured photokinetically with the aid of bacterial luciferase.ICDH (NAD) and FI.I displayed similar activities in the skin of normal controls to those in the non-involved skin of the patients with various dermatoses. In the psoriatic lesion both enzymatic activities were markedly increased in the two layers studied. In neurodermatitis and lichen planus, however, the ICDI-I(NAD) activity was increased only in basal epidermis. FH activities were not increased over control levels in any of the three dermatoses. In epidermis ICDH(NADP) showed 20-fold higher activity than ICDI-I(NAD). The activity was similar in controls and in the non-involved skin of the patients with tile 4 dermatoses as well as in the lesions of pityriasis rosea. It was increased 60~ and 30a/o in basal layers of the psoriatic and the neurodermite lesions, respectively. The enzymatic activity was decreased 50 ~ in subcorneal layers of lichen planus.In various tissues ICDH (NAD) is considered to be a strictly intramitochondrial enzyme, whereas ICDH(NADP) and FH are found also in the cytosol. If this also applies to epidermis, the results imply differences of mitochondrial function in psoriasis and the contrasted dermatoses. The high activiW of ICDH (NAD) in parakeratosis (psoriasis) as compared to that in orthokeratosis (lichen planus) was also discernable in neurodermatitis when para-and orthokeratotic epidermis were analysed separately. Zusammen

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Section: Selection El Neurodermite Materials For Icdh (Nad)supporting

confidence: 80%

NAD and NADP dependent isocitrate dehydrogenase and fumarate hydratase activities in normal human skin and in some maculosquamous diseases of the skin

Hammar

1973

Arch. Derm. Res.

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“…It is therefore tempting to suggest that the most heat sensitive step in the energy producing processes of skin lies in the Krebs' cycle. Hershey et al (1965), in a study of the effects of heat on enzymes of skin homogenates, reported that fumarase is particularly heat labile, activity being reduced by 70% after 5 min heating at 45°C. However, although there is now little doubt that a functional Krebs' cycle exists in skin (Cruickshank, 1964) its relative importance is not established.…”

Section: Discussionmentioning

confidence: 99%