A Gram-stain-negative, aerobic, motile, short-rod-shaped bacterium with nicosulfuron-degrading ability, designated strain LAM1902T, was isolated from a microbial consortium enriched with nicosulfuron as a sole nitrogen and energy source. The optimal temperature and pH for growth of strain LAM1902T were 30 °C and pH 6.0, respectively. Strain LAM1902T could grow in the presence of NaCl with concentration up to 4.0 % (w/v). Comparative analysis of 16S rRNA gene sequences revealed that LAM1902T was closely related to the members of the family Pseudomonadaceae to the genus Pseudomonas , with the highest similarity to Pseudomonas nitroreducens DSM 14399T (99.6 %), Pseudomonas nitritireducens WZBFD3-5A2T (99.3 %) and Pseudomonas panipatensis Esp-1T (98.8 %). Multi-locus sequence analysis based on both concatenated sequences of the 16S rRNA gene and three housekeeping genes (gyrB, rpoB and rpoD) further confirmed the intrageneric phylogenetic position of strain LAM1902T. The genomic DNA G+C content of LAM1902T was 64.8 mol%. The low values of in silico DNA–DNA hybridization (less than 43.7 %) and average nucleotide identity (less than 90.9 %) also showed that the strain was distinctly different from known species of the genus Pseudomonas . The major fatty acids were C16 : 0, C17 : 0 cyclo and anteiso C15 : 0. Ubiquinone Q-9 was detected as the predorminant respiratory quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on phylogenetic, phenotypic and chemotaxonomic analyses and genome comparisons, we conclude that strain LAM1902T represents a novel species, for which the name Pseudomonas nicosulfuronedens sp. nov. is proposed. The type strain is LAM1902T (=JCM 33860T=KCTC 72830T).

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A novel Gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7116 T , was isolated from a sulfonylurea herbicides degrading consortium enriched with the birch forest soil from Xinjiang. The optimal temperature and pH for the growth of strain LAM7116 T were 35 °C and 7.0, respectively. Strain LAM7116 T could grow in the presence of NaCl up to 4% (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain LAM7116 T was closely related to the members of the genus Microbacterium, with the highest similarity to Microbacterium avescens DSM 20643 T (98.1%) and Microbacterium kitamiense Kitami C2 T (98.1 %). Strain LAM7116 T formed a distinct subclade with M. avescens DSM 20643 T within the genus Microbacterium in the 16S rRNA gene phylogenetic trees. The genomic DNA G+C content of LAM7116 T was 69.9 mol%. The DNA-DNA hybridization (DDH) value between strain LAM7116 T and M. avescens DSM 20643 T was 27.20 %. The average nucleotide identity (ANI) value was 83.96 % by comparing the draft genome sequences of strain LAM7116 T and M. avescens DSM 20643 T . The major fatty acids were anteiso-C 15:0 , anteiso-C 17:0 , iso-C 17:0 and iso-C 16:0 . The respiratory quinones of strain LAM7116 T were MK-13 and MK-14. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidenti ed lipid and an unidenti ed glycolipid. Based on the phenotypic characteristics and genotypic analyses, we propose that strain LAM7116 T represents a novel species, for which the name Microbacterium sulfonylureivorans sp. nov. was proposed. The type strain is LAM7116 T (=CGMCC 1.16620 T =JCM 32823 T ). Strain LAM7116 T secreted auxin IAA and grew well in Ashby nitrogen-free culture medium. Genomic results showed that strain LAM7116 T carried the nitrogenase iron protein (nifU and nifR3) gene, which indicated that strain LAM7116 T has the potential to x nitrogen and promote plant growth. At the same time, the strain LAM7116 T can degrade nicosulfuron (a kind of sulfonylurea herbicides) by using glucose as carbon source, microbial degradation of nicosulfuron is the primary process of removing nicosulfuron from environments, and biodegradation is the most effective and environmentally friendly. Microbacterium sulfonylureivorans sp. nov. LAM7116 T is a potential candidate for the biofertilizers of organic agriculture areas, having broad prospects for using, and may possess potential to be used in bioremediation of nicosulfuron-contaminated environments.

A novel Gram-stain-positive, aerobic, non-motile and rod-shaped bacterium, designated strain NC76-1T, was isolated from soil from a field that had undergone seven years continuous maize cropping from Liuba town located in Zhangye city, Gansu province, PR China. Colonies of strain NC76-1T were white, opaque and circular with a convex shape. The isolate was found to be able to grow at 10–40 °C (optimum 30 °C), pH 6.0 to 12.0 (optimum 7.0–8.0) and with 0–5.0 % (w/v) NaCl (optimum 0%). On the basis of the results of 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter , showing the highest sequence similarities with Leucobacter iarius 40T (97.4%), Leucobacter aridicollis CIP 108388T (97.0%), Leucobacter chromiireducens subsp. solipictus TAN 31504T (96.7%) and Leucobacter denitrificans M1T8B10T (96.7%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between NC76-1T and its closest relatives, L. iarius 40T, L. aridicollis CIP 108388T, L. chromiireducens subsp. solipictus TAN 31504T and L. denitrificans M1T8B10T were ≤73.5 % and 20.3%, respectively. The genomic DNA G+C content of NC76-1T was 61.5 mol%. It presented MK-11 as the predominant menaquinone. The major cellular fatty acids were anteiso-C15 : 0 (49.2 %) and iso-C16 : 0 (35.7%). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycolipid, five glycolipid and one unidentified lipids. The cell wall amino acids were 2,4-diaminobutyric acid, alanine, glutamic acid, glycine and threonine. On the basis of the phylogenetic, phenotypic and chemotaxonomic characteristics, strain NC76-1T is concluded to represent a novel species within the genus Leucobacter , for which the name Leucobacter chinensis sp. nov. is proposed. The type strain is NC76-1T (GDMCC 1.2286T= JCM 34651T).