Tithonia diversifolia es una planta con alto potencial para la alimentación animal. El objetivo del presente trabajo fue evaluar el efecto de diferentes materiales vegetales (mv) en la población de metanógenos y protozoos del rumen, se empleó la técnica in vitro de producción de gases. Los tratamientos consistieron en los siguientes materiales vegetales de T. diversifolia: mv-3, mv-5, mv- 6, mv-10, mv- 13, mv-17, mv-23, mv-24 y mv-25. Éstos se compararon con un tratamiento control de pasto estrella (Cynodon nlemfuensis). Los muestreos se realizaron a las 3 horas posteriores a la incubación y se replicó 4 veces. El diseño experimental fue completamente aleatorizado. Los valores de proteína cruda (PC) oscilaron desde18, 26 en el mv- 3 hasta 26,40 para el caso del mv- 26. Las poblaciones de metanógenos fueron 27,7; 23,5; 21,3; 16,2; 20,0; 19,4; 12,4; 22,5 y 20,2. 1010 UFC/mL para pasto estrella y los mv de T. diversifolia 3, 5, 6, 10, 13, 17, 23, 24 y 25, respectivamente. Las poblaciones de protozoos en el tratamiento control fueron de 48. 105células/mLy 14; 11; 10; 7; 10; 9; 4; 10 y 9, para los mv -3, mv-5, mv-6, mv-10, mv-13, mv-17, mv-23, mv-24 y mv-25, respectivamente. Se destacan como los más promisorios para reducir los metanógenos y protozoos del rumen, los mv- 23 y mv -10. Se concluye que los materiales vegetales de T. diversifolia ejercen efecto depresivo en las poblaciones de metanógenos y protozoos y se destacan los mv -23 y mv-10 como los más promisorios para estos propósitosPalabras clave: rumen, metanogénesis, titonia ABSTRACTTithonia diversifolia is a plant with high potential for animal feed. The objective of the present work was to evaluate the effect of different plant materials (vm) in the population of methanogens and rumen protozoa, the in vitro technique of gas production was used. The treatments consisted of the following plant materials of T. diversifolia: mv-3, mv-5, mv-6, mv-10, mv-13, mv-17, mv-23, mv-24 and mv-25. These were compared with a control treatment of star grass (Cynodon nlemfuensis). Samples were taken at 3 hours after incubation and replicated 4 times. The experimental design was completely randomized. Crude protein (CP) values ranged from 18.26 in mv-3 to 26.40 in the case of mv-26. The methanogen populations were 27.7; 23.5; 21.3; 16.2; 20.0; 19.4; 12.4; 22.5 and 20.2. 1010 CFU / mL for star grass and the mv of T. diversifolia 3, 5, 6, 10, 13, 17, 23, 24 and 25, respectively. The protozoa populations in the control treatment were 48. 105 cells / mL and 14; 11; 10; 7; 10; 9; 4; 10 and 9, for mv -3, mv-5, mv-6, mv-10, mv-13, mv-17, mv-23, mv-24 and mv-25, respectively. They stand out as the most promising to reduce the methanogens and protozoa of the rumen, the mv-23 and mv -10. It is concluded that the plant materials of T. diversifolia exert a depressive effect on the populations of methanogens and protozoa and the mv -23 and mv-10 stand out as the most promising for these purposes.Keywords: rumen, methanogenesis, titonia
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