BACKGROUND: Peronema canescens (Sungkai) leaves have been popular in Indonesia which contain various bioactive compounds with empirical therapeutic efficacy in dealing with COVID-19 and various other diseases. Total phenolic and flavonoid content and antioxidant activity using the DPPH method from P. canescens leaf extract have not been studied much. AIM: This research has several objectives. The first is to compare the results of qualitative phytochemical analysis of the ethanol extract of the leaves of P. canescens (EEPL). The second is to measure the total phenol and flavonoid content. The third is to test the FTIR and antioxidant activity of the ethanol extract of P. canescens leaves in vitro using the DPPH method. METHODS: Fresh plant material and simplicia, ethanol extract extracted by maceration method using 96% ethanol as solvent from P. canescens. The Dragendorff’s and Mayer test carried out the qualitative phytochemical analysis, FeCl3 test, Salkowski method, Liebermann–Burchard method, foam test, and NaOH reagent. The total phenolic and flavonoid levels were tested using the Folin–Ciocalteu method. In vitro antioxidant activity was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. RESULTS: The results of qualitative phytochemical screening showed that alkaloids, flavonoids, saponins, tannins, and steroids were detected in the extract of P. canescens. The spectra from the FTIR test results showed various absorbance peak values indicating the bonding of specific functional groups, namely: 418.12, 599.94, 666.67, 1036.39, 1159.52, 1224.16, 1348.95, 1454.19, 1600.87, 1732.00, 2923.13, and 3353.01 cm-1. In the test results, total phenolic content was as much as 5.64% (mgEAG/g) and total flavonoid content of 142,247 mgEQ/g in a sample of 1 mg extract, which was equivalent to 1 mg quercetin. EEPL has antioxidant activity with the DPPH IC50 method of 116.7865 ppm. CONCLUSION: The data obtained at this time can contribute to the exploitation of P. canescens leaves in the future as one of the nutraceutical products, supplements, and herbal medicines by specific industries related to improving the health status of the world community. The higher the bioactive substance in preparation, the more significant the effect of the pharmacological efficacy response. P. canescens ethanol extract has good total phenolic content, total flavonoid content, and antioxidant action.
Latar belakang : Pasien kanker stadium lanjut pada umumnya mengalami kurang gizi, karena terjadinya hipermetabolisme dan pengaruh terapi kemoradiasi. Diet modifikasi dengan porsi kecil dan mengandung sumber peptida, BCAA, omega3 dan MCT diharapkan bisa mencegah penurunan berat badan, mengurangi anoreksia dan inflamasi. Tujuan penelitian menganalisis pengaruh pemberian diet modifikasi terhadap kenaikan status gizi pasien kanker. Metode : Rancangan penelitian adalah Randomised Control Trial dengan pre post-test control group design. Populasi dalam penelitian ini adalah pasien rawat inap kanker dengan kemoradiasi di RSUP Dr. Kariadi bulan Juni–September 2013. Subjek sebanyak 66 orang dipilih secara consecutive sampling, dibagi 2 kelompok secara random. Kelompok perlakuan diberi diet modifikasi, kelompok kontrol diberi diet standar rumah sakit. Sebelum dan setelah intervensi 21 hari diukur data antropometri (berat badan, indeks massa tubuh, lingkar lengan atas dan triceps skinfold) dan biokimia (kadar albumin, hemoglobin, jumlah lekosit dan total lymphocite count) serta asupan makanan. Analisis statistik dengan Paired Samples T Test, Mann Whitney Test, Independent Samples T Test dan Wilcoxon Rank Test. Analisis multivariat dilakukan dengan ancova pada tingkat kemaknaan p<0,05. Hasil : Diet modifikasi meningkatkan berat badan (p=0,001), indeks massa tubuh (p=0,001), kadar albumin (p=0,001), kadar hemoglobin (p=0,007), jumlah lekosit (p=0,042) dan total lymphocite count (p=0,001) pada kelompok perlakuan. Pada kelompok kontrol terjadi penurunan berat badan (p=0,002), indeks massa Tubuh (p=0,002), kadar albumin (p=0,001), kadar hemoglobin (p=0,001), jumlah lekosit (p=0,001) dan total lymphocyte count (p=0,001) setelah intervensi. Diet modifikasi meningkatkan berat badan (p=0,024), indeks massa tubuh (p=0,028), kadar albumin (p=0,007), kadar hemoglobin (p=0,002) dan total lymphocyte count (p=0,001) setelah dikontrol dengan asupan protein. Simpulan : Pemberian diet modifikasi selama 21 hari meningkatkan berat badan, indeks massa tubuh, kadar albumin, kadar hemoglobin dan total lymphocyte count pada pasien rawat inap kanker dengan kemoradiasi
ABSTRAKKacang komak berpotensi sebagai pangan alternatif pengganti kedelai dengan kandungan gizi yang tidak jauh berbeda, tetapi kadar lemak yang jauh lebih rendah. Yoghurt kacang komak adalah produk susu fermentasi dengan bakteri Lactobacillus bulgaricus dan Streptococcus thermophillus, sehingga laktosa diubah menjadi asam laktat dan diperoleh keasaman, bau, dan rasa yang khas. Tujuan penelitian adalah mengetahui pengaruh pemberian substitusi sari kacang komak dan susu skim terhadap sifat organoleptik, nilai pH, dan total bakteri asam laktat (BAL). Desain penelitian yaitu rancangan acak lengkap dengan sampel sebanyak 30 panelis. Penelitian dilakukan sebanyak 3 perlakuan dengan 2 pengulangan. Data yang dikumpulkan adalah total BAL dan nilai pH. Uji sifat organoleptik menggunakan uji mutu hedonik dengan 1 pengulangan. Nilai pH diukur menggunakan pH meter elektronik. Total BAL diukur menggunakan media Man Rogosa and Sharpe (MRS). Kadar asam laktat diuji menggunakan uji keasaman. Perlakuan substitusi sari kacang komak dilakukan dengan 92 %, 95%, dan 96,5%, dengan susu skim 8%, 5% dan 3,5%. Data dianalisis dengan Anova, Kruskal Wallis, Duncan Multi Range tingkat kemaknaan p < 0,05. Hasil menunjukkan bahwa sifat organoleptik substitusi 92:8 lebih mendekati kualitas yoghurt yang diharapkan dengan nilai pH terendah, serta nilai pH tertinggi terdapat pada substitusi 96,5:3,5. Total BAL dan kadar asam laktat menunjukkan substitusi 92:8 memiliki nilai tertinggi dan substitusi 96,5:3,5 nilai terendah. Hasil mikroskopis pewarnaan Gram terdapat bakteri Streptococcus thermopillus, dan Lactobacillus bulgaricus pada yoghurt kacang komak. Kacang komak berpotensi sebagai bahan utama pembuatan yoghurt. Kata kunci : bakteri asam laktat, organoleptik, pH, yoghurt kacang komak ABSTRACT Lablab bean has potential as alternative food subtitute of soybean Lablab Bean has nutrients content which is not much different, but fat content is much lower than soybean. Yogurt is milk fermented by the lactic acid bacteria Streptococcus thermophilus and Lactobacillus bulgaricus that tranform milk sugar (lactose) into lactic acid to obtain acidity, smell, and specifi c fl avor. The aim of this research was to study the effect of substitution of lablab bean milk and skim milk to organoleptic properties, pH value, and the total of lactic acid bacteria (LAB). The Experimental design was applied
Background (10,95 ± 0,71 mg/dl) to (11,81 ±0,53 mg/dl)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.