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The exposure to aflatoxin B, (AFB) in animal-feed processing plants was assessed using bindingofAFB to serum albumin. The albumin fraction was digested with pronase, and the digest was purified on a C18 Sepak column and an aflatest affinity colunm before quantification by ELISA. The level ofdetectability was5 pg/mg albumin. The workers served as their own controls, as blood samples were taken upon return from vacation and after 4 weeks ofwork. A total of 7 of45 samples were positive for AFB, with an estimated average daily intake of 64 ng AFB/kg body weight. The exposed workers had been disembarking cargos contaminated with AFB or working at places where the dust contained detectable amounts of AFB. The sera from the exposed workers had a significantly higher titer against an aflatoxin BI-epitope than a nonexposed Danish control group. The level ofexposurecould partly explain the increased risk of liver cancer in workers in the animalfeed processing industry.