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For the investigation of locoregional chemotherapy of liver neoplasms we developed a standardized animal model in the rat. Continuous infusion therapy or repeated bolus injections of FUDR or 5-FU were given via the hepatic artery, the portal vein or the vena cava in tumor-bearing animals. The efficacy of the treatment was determined by measuring the tumor volume 3 weeks after tumor cell implantation. For the evaluation of the local and systemic toxicity serum GOT, GPT, and total bilirubin were determined. DNA single strand breaks were assessed in isolated liver and bone marrow cells. Inhibition of colony formation of bone marrow stem cells was determined by CFU-C and CFU-S bioassay. A significant reduction of tumor growth was observed only after continuous infusion of FUDR via the hepatic artery. Systemic toxicity was lowest in this group for both compounds while the local liver toxicity was only slightly elevated.

Infestação mista por Lynxacarus radovskyi e Felicola subrostratus em um gato na região de Porto Alegre, RS, Brasil. Acta Scientiae Veterinariae. 37(3): 301-305.A linxacariose é uma doença incomum que acomete felinos, principalmente em regiões tropicais, porém há registros também em regiões subtropicais. No Brasil, foi descrita pela primeira vez em 1986, e atualmente existem relatos em todas as regiões, excluindo o centro-oeste do país. No Rio Grande do Sul, somente alguns casos foram relatados no ano de 1997 em felinos das raças persa e exótico. Esta doença é causada pela infestação do ácaro Lynxacarus radovskyi. Algumas vezes, esta infestação pode estar associada a outras parasitoses externas, na qual a mais comum é a pediculose por Felicola subrostratus, parasita de distribuição mundial. Geralmente, a transmissão é dada por contato direto ou por fômites, mas não é considerada uma doença de alto contágio. Os animais infestados apresentam variado grau de prurido, alopecia, disqueratinização, pelagem opaca e pelos aparentemente com sujidades, tradicionalmente com o aspecto "sal e pimenta". Algumas vezes, a manifestação clínica da doença pode ser confundida com outras dermatopatias, não sendo corretamente diagnosticada. O diagnóstico é dado pela visualização direta do ácaro e por microscopia óptica. O objetivo do relato é descrever um caso de infestação ABSTRACT Lynxacariosis is an uncommon parasitic disease in felines recorded in tropical, and sometimes subtropical, regions of varied countries. In Brazil lynxacariosis was first described in 1986, in Rio de Janeiro, and currently there are reports in all regions of the country, excluding the central-west region. In Rio Grande do Sul, only a few cases were reported in 1997 in Persian Exotic cats. The disease is caused by the infestation of the mite Lynxacarus radovskyi. Sometimes this infestation can be mixed with other external parasitoses, most commonly with the pediculosis caused by Felicola subrostratus, a parasite distributed worldwide. Usually the transmission occurs by direct contact or by fomites, but it is not considered as a highly transmissible disease. The infested animals present variable degree of pruritus, alopecia, scalling, and a "salt and pepper" appearance to the dull and dirty coat. Sometimes the clinical manifestation of the disease can be confused with other dermatitis, not being correctly diagnosed. The diagnosis is confirmed by the direct visualization of the mite and by optic microscopy. The objective of this article is to report a case of infestation by L. radoviskyi associated with F. subrostratus in a mixed cat, attended

Background:The Shaker Dog Syndrome manifests itself as generalized tremors that usually affect the head and body of the patient. The tremors increase with movement and decrease at rest, and it may cease during sleep. The disorder develops mostly in 1-year to 5-year-old animals weighing below 15 kg. The diagnosis is based on the exclusion of other possible causes of tremors and a positive response to treatment with corticosteroids. The treatment consists of immunosuppressant doses of corticosteroids and it can be associated to decreasing diazepam doses during 8 to 12 weeks. Literature data are very rare and cannot be found in Brazil. Consequently, this study aimed at describing a Shaker Dog Syndrome case that is responsive to corticosteroids. Case: A 2-year-old mongrel female dog, weighting 7.5 kg, was referred to the Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul, presenting generalized tremors for 5 days, which would cease during sleep. In the neurological examination, the cranial nerve exam and the test for postural reactions were considered normal. The hematological and biochemical profile did not show alterations. The cervical spine radiography did not display bone alterations. A density of 1.016 was observed at the physical examination of the cerebrospinal fluid. The chemical examination revealed a glucose level of 100 mg/dL, negative bilirubin, negative ketones, absence of occult blood, pH 8.0, presence of protein traces, normal urobilinogen, negative reactive protein C (PCR), normal creatine kinase (CK) and aspartate aminotransferase (AST). The result of Pandy's test was negative. Oral treatment with prednisone at a dose of 1.5 mg.kg -1 BID and diazepam at a dose of 0.5 mg.kg -1 BID was instituted, this latter during 4 days. The corticoid dose was maintained until total disappearance of the tremors, which occurred within 24 days. Afterwards, a gradual reduction of 50% of the dose was made every 7 days, during 4 weeks. Discussion: During anamnesis, tremors induced by trauma and medication were ruled out. Myelin abnormalities were also improbable, as they cause congenital tremors and do not quickly respond to corticosteroids. A colorless aspect and absence of occult blood and bilirubin was observed in the LCR analysis. The absence of turbidity and the presence of protein traces in this sample demonstrate normality (38.7 mg/dL ± 15.49). Chemical and physical exams did not demonstrate alterations -pH 8.0 (8.30 ± 0.34) and a density of 1.016 (1007 ± 1.83), respectively. The glucose concentration in the cerebrospinal fluid was not reduced. The results obtained from Pandy's and reactive protein C tests were negative. The analysis of AST and CK enzymes was normal. Since the values obtained from enzymatic and protein evaluations of the cerebrospinal fluid were normal, they suggest an absence of infectious and degenerative processes. The immunosuppressant prednisone dose recommended by literature is variable; thus, the smallest corticosteroid dose indicated was selected, which is 1.5 ...

Displasia renal é a desorganização estrutural do parênquima renal durante a embriogênese, induzindo ao desenvolvimento de insuficiência renal crônica em cães filhotes e adultos jovens. Este trabalho tem por objetivo relatar um caso de displasia renal em um canino, macho, sem raça definida, com 1 ano e 2 meses de idade, apresentando sinais clínicos de insuficiência renal crônica. Ao exame laboratorial, verificou-se anemia arregenerativa e azotemia. À ultrassonografia, observou-se hipotrofia renal com perda de definição córtico-medular e hiperecogenicidade do parênquima renal. À necropsia, constataram-se rins pequenos, irregulares e de coloração pálida. Ao exame histológico, revelou-se glomérulos e túbulos imaturos, tecido mesangial persistente, dilatação tubular, material eosinofílico no interior de túbulos, mineralização na região medular e fibrose, confirmando o diagnóstico de displasia renal.