Penelitian ini bertujuan untuk mengetahui pengaruh perendaman bakteriosin asal Streptococcus bovis isolat 9A sebagai biopreservatif pada daging sapi ditinjau dari uji Eber. Penelitian dimulai dari persiapan kultur isolat 9A dan dilanjutkan dengan produksi bakteriosin kasar, persiapan sampel dan aplikasi bakteriosin sebagai biopreservatif. Hasil penelitian menunjukkan bahwa perendaman daging dalam bakteriosin 5 menit, 10 menit dan tanpa bakteriosin berpengaruh terhadap kualitas daging berdasarkan uji Eber. Pada penelitian ini dapat disimpulkan bahwa perendaman daging dalam bakteriosin asal Streptococcus bovis isolat 9A mampu memperpanjang masa simpan daging sapi.

Aim: This study aimed to determine the effect of the bacteriocin produced by strain 9 lactic acid bacteria (LAB) isolate on the biopreservation of beef. Materials and Methods: The strain 9 LAB isolate was identified conventionally by culturing with de Man, Rogosa, and Sharpe broth medium followed by Gram staining and catalase testing. The molecular confirmation of the isolate involved analyzing the 16S rRNA gene with specific primers, that is, B27F (5-AGAGTTTGATCCTGGCTCAG-3) and U1492R (5-GGTTACCTTGTTACGACTT-3). Then, the isolate was centrifuged to evaluate the bacteriocin production, and the effect of the biopreservative activity in beef was evaluated by measuring the NH3 produced with the Eber test and the organoleptic acceptance from expert panels. Results: This study confirmed that the strain 9 LAB isolate was a strain of Pediococcus pentosaceus, and the bacteriocin product showed biopreservative potential. The biopreservative potential was characterized by a significant decline in the production of NH3 and the panel's acceptance of the texture and tenderness of the beef, compared with the control, after 10 days of constant treatment. Conclusion: This study highlighted the high biopreservative potency of pediocin produced by P. pentosaceus strain 9. This was noted by the production of NH3 and the modifications in texture and tenderness.