Glucosinolate (GSL) profiles and concentrations in various tissues (seeds, sprouts, mature root, and shoot) were determined and compared across nine Brassica species, including cauliflower, cabbage, broccoli, radish, baemuchae, pakchoi, Chinese cabbage, leaf mustard, and kale. The compositions and concentrations of individual GSLs varied among crops, tissues, and growth stages. Seeds had highest total GSL concentrations in most of crops, whereas shoots had the lowest GSL concentrations. Aliphatic GSL concentrations were the highest in seeds, followed by that in sprouts, shoots, and roots. Indole GSL concentration was the highest in the root or shoot tissues in most of the crops. In contrast, aromatic GSL concentrations were highest in roots. Of the nine crops examined, broccoli exhibited the highest total GSL concentration in seeds (110.76 µmol·g ). The lowest GSL concentrations were observed in radish across all tissues examined.

Chlorophyll fluorescence (CF), growth parameters, phytochemical contents [proline, chlorophyll, ascorbic acid, total phenol content (TPC), total flavonoid content (TFC)], and antioxidant activities were investigated in lettuce (Lactuca sativa L.) seedlings grown under different sodium chloride (NaCl) concentrations (0, 50, 100, 200, 300, and 400 mM) in a controlled environment for eight days. The parameters were evaluated at two days intervals. Almost of the CF and growth parameters as well as phytochemicals were significantly affected by both NaCl concentrations and progressive treatment schedule. The maximum quantum yield (Fv/Fm), effective quantum yield of photochemical energy conversion in PSII [Y(PSII)], coefficient of photochemical quenching (qP), coefficient of non-photochemical quenching (qN), and ratio of fluorescence decline (Rfd) showed decrements only at the highest saline concentration (400 mM), whereas the quantum yield of non-regulated energy dissipation in PSII [Y(NO)] exhibited a dissipation trend. All the growth parameters decreased with increasing NaCl concentrations, showing the highest decrease (~8 fold) in shoot fresh weight, compared to control seedlings. Proline significantly increased with increasing NaCl concentration and treatment time. Other phytochemicals decreased with the increase in NaCl concentration and reached their lowest at 400 mM. Overall, the results showed major changes in all parameters when the seedlings were grown at a NaCl concentration of 400 mM. The present findings will be useful for understanding the differential effect of NaCl concentrations in lettuce seedlings, and also might be useful to optimize the NaCl concentrations in other crops grown in controlled environmental conditions.

This study monitored changes in chlorophyll fluorescence (CF), growth parameters, soil moisture content, phytochemical content (proline, ascorbic acid, chlorophyll, total phenol content (TPC), and total flavonoid content (TFC)), and antioxidant activities in 12-day-old lettuce (Lactuca sativa L.) seedlings grown under drought stress (no irrigation) and control (well irrigated) treatments in controlled conditions for eight days. Measurements occurred at two-day intervals. Among ten CF parameters studied, effective quantum yield of photochemical energy conversion in PSII (Y(PSII)), coefficient of photochemical quenching (qP), and coefficient of photochemical quenching of variable fluorescence based on the lake model of PSII (qL) significantly decreased in drought-stressed seedlings from day 6 of treatment compared to control. In contrast, maximum quantum yield (Fv/Fm), ratio of fluorescence (Rfd), and quantum yield of non-regulated energy dissipation in PSII (Y(NO)) were significantly affected only at the end. All growth parameters decreased in drought-stressed seedlings compared to control. Proline started increasing from day 4 and showed ~660-fold elevation on day 8 compared to control. Chlorophyll, ascorbic acid, TPC, TFC, and antioxidant activities decreased in drought-stressed seedlings. Results showed major changes in all parameters in seedlings under prolonged drought stress. These findings clarify effects of drought stress in lettuce seedlings during progressive drought exposure and will be useful in the seedling industry.

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Individual glucosinolates (GSLs) were assessed to select cabbage genotypes for a potential breeding program. One hundred forty-six cabbage genotypes from different origins were grown in an open field from March to June 2019; the cabbage heads were used for GSL analyses. Seven aliphatics [glucoiberin (GIB), progoitrin (PRO), epi-progoitrin (EPI), sinigrin (SIN), glucoraphanin (GRA), glucoerucin (GER) and gluconapin (GNA)], one aromatic [gluconasturtiin (GNS)] and four indolyl GSLs [glucobrassicin (GBS), 4-hydroxyglucobrassicin (4HGBS), 4-methoxyglucobrassicin (4MGBS), neoglucobrassicin (NGBS)] were found this study. Significant variation was observed in the individual GSL content and in each class of GSLs among the cabbage genotypes. Aliphatic GSLs were predominant (58.5%) among the total GSLs, followed by indolyl GSL (40.7%) and aromatic GSLs (0.8%), showing 46.4, 51.2 and 137.8% coefficients of variation, respectively. GIB, GBS and NGBS were the most common GSLs found in all genotypes. GBS was the most dominant GSL, with an average value of 3.91 µmol g−1 (0.79 to 13.14 µmol g−1). SIN, GIB, PRO and GRA were the other major GSLs, showing average values of 3.45, 1.50, 0.77 and 0.62 µmol g−1, respectively. The genotypes with relatively high contents of GBS, SIN, GIB and GRA warrant detailed studies for future breeding programs since the hydrolysis products of these GSLs have several anti-cancer properties.