A increasing number of microRNAs have been shown to play important regulatory roles in plant responses to various metal stresses. However, little information about miRNAs especially miRNAs responsive to cadmium (Cd) stress is available in wheat. To investigate the role of miRNAs in responses to Cd stress, wheat seedlings were subjected to 250 μM Cd solution for 6, 12, 24 and 48 h, and analyses of morphological and physiological changes as well as the expression of five miRNAs and their corresponding targets were carried out. Our results demonstrated that miRNAs and their targets were differentially expressed in leaves and roots of wheat seedlings exposed to Cd stress. Furthermore, miR398 may involve in oxidative stress tolerance by regulating its target CSD to participate in Cd stress. Among ten miRNA-target pairs studied, nine pairs showed complex regulation relationship in leaves and roots of wheat seedlings exposed to Cd stress. These findings suggested that miRNAs are involved in the mediation of Cd stress signaling responses in wheat. The characterization of the miRNAs and the associated targets in responses to Cd exposure provides a framework for understanding the molecular mechanism of heavy metal tolerance in plants.
In low-Earth orbit, the already existing population of small and medium debris (between 1 cm and several dozens of cm) is a concrete threat to operational satellites. A space-based laser space debris removal (SLDR) system that can remove hazardous debris around selected space assets appears to be a flexible and effective project. To achieve high-precision tracking and emitting, the optical system of the SLDR mission includes a target-detection telescope and emitting telescope, adopting a common light path structure. The optical design results, system performance, tolerance budget, and detailed stray light control design are presented in this paper. The large-aperture off-axis two-mirror beam-narrowing system characteristics are also discussed in terms of stray light control. This paper will present the lateral-displacement (LD) setting, two-stage fore baffle design, black baffle surface selection, and opening direction of the telescope door. The results showed that the stray light elimination reaches a
10
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order, meeting design requirements.
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