OBJECTIVE To assess the feasibility of blood oxygen level–dependent (BOLD) MRI for measurement of the renal T2* relaxation rate (R2*; proxy for renal oxygenation) before and after furosemide administration and to evaluate the reliability and repeatability of those measurements in healthy dogs. ANIMALS 8 healthy adult Beagles (4 males and 4 females). PROCEDURES Each dog was anesthetized and underwent BOLD MRI before (baseline) and 3 minutes after administration of furosemide (1 mg/kg, IV) twice, with a 1-week interval between scanning sessions. Mapping software was used to process MRI images and measure R2* and the difference in R2* (∆R2*) before and after furosemide administration. The intraclass correlation coefficient was calculated to assess measurement reliability, and the coefficient of variation and Bland-Altman method were used to assess measurement repeatability. RESULTS Mean ± SD baseline R2* in the renal medulla (24.5 ± 3.8 seconds−1) was significantly greater than that in the renal cortex (20.6 ± 2.7 seconds−1). Mean R2* in the renal cortex (18.6 ± 2.6 seconds−1) and medulla (17.8 ± 1.5 seconds−1) decreased significantly after furosemide administration. Mean ∆R2* in the medulla (6.7 ± 2.4 seconds−1) was significantly greater than that in the renal cortex (2.1 ± 0.7 seconds−1). All R2* and ∆R2* values had good or excellent reliability and repeatability, except the cortical ∆R2*, which had poor repeatability. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that BOLD MRI, when performed before and after furosemide administration, was noninvasive and highly reliable and repeatable for dynamic evaluation of renal oxygenation in healthy dogs.

With the increasing use of radiation therapy for treatment of canine sinonasal neoplasia, there is a need for developing low-dose CT protocols to help minimize radiation exposure. The purpose of this study was to assess the trade-off between image quality and reduced radiation exposure of a low-dose CT technique in the canine sinonasal cavity. In this prospective, experimental study, CT images of the sinonasal cavities from 10 normal Beagles were acquired using high-dose (130 kVp) or low-dose (110 kVp, 80 kVp) protocol. Radiation dose and image quality were compared. Radiation exposure measured by the volume-weighted CT dose index and dose-length product was reduced by 36% at 110 kVp and 74% at 80 kVp respectively, compared to the corresponding values at 130 kVp (P = 0.000). Low-dose protocol resulted in higher image noise and reduced signal-to-noise ratio and contrast-to-noise ratio than 130 kVp in most evaluated regions of interest (P < 0.05). CT numbers of the contrast-enhanced structures were highest at 80 kVp (P = 0.000). Conspicuity of most sinonasal structures was similar for high dose and both lower dose protocols. The results of this study indicate that 80 or 110 kVp can be used for sinonasal CT examinations to reduce radiation exposure to the patient without compromising image quality.

Endoscopic ultrasound (EUS) is a medical procedure in which endoscopy is combined with ultrasonography (US) to compensate for problems associated with the transabdominal US such as large penetration depths, presence of intestinal gas, and acoustic shadowing. This prospective, method comparison, pilot study was performed to assess the feasibility of applying EUS in the colorectal region and to describe the typical EUS features of the descending colon and rectum in healthy dogs. Transabdominal US and EUS with or without the hydrosonography were applied to the descending colon and rectum in 10 clinically healthy Beagle dogs and wall thickness, visibility of the wall layers, and conspicuity of the mucosal and serosal surfaces of the intestinal wall were assessed. Endoscopic ultrasound enabled circumferential evaluation of the colorectal wall and provided better visibility of the wall layers and conspicuity of the mucosal and serosal surfaces without degradation of the image, even in the far‐field portion of the colorectal wall, compared to US. Moreover, EUS provided the adequate image quality of the rectum, which was difficult to evaluate with US due to deep scan depth and acoustic shadowing by the pelvis. Meanwhile, the application of hydrosonography to EUS deteriorated the visibility of the wall layers and conspicuity of the intestinal wall. The results of this study demonstrate the feasibility of EUS to assess the colorectal region and its potential application for the evaluation of rectal masses or intrapelvic lesions that are inaccessible by the transabdominal US in dogs.

Blood supply to the peripheral nerves is essential for fulfilling their structural and functional requirements. This prospective, experimental, exploratory study aimed to assess the feasibility of contrast-enhanced ultrasonography (CEUS) for evaluating blood perfusion of the sciatic nerve in normal dogs. Contrast-enhanced ultrasonography examinations were performed on the bilateral sciatic nerves after bolus injection of Sonazoid™ (0.015 mL/kg) in 12 healthy Beagles for 150 s. Then, qualitative assessment of the wash-in timing, degree and enhancement patterns, and quantitative measurement of the peak intensity and time to peak intensity were performed from the sciatic nerve. The results were compared to those obtained from the adductor muscle around the nerve and caudal gluteal artery. After contrast agent injection, the sciatic nerve was enhanced at approximately 13-14 s, immediately after wash-in of the caudal gluteal artery. The peak intensity of the sciatic nerve was significantly lower than that of the caudal gluteal artery and higher than that of the adductor muscle. The time to peak intensity was significantly slower than that of the caudal gluteal artery; but was not significantly different from that of the adductor muscle. There were no significant differences in the peak intensity and time to peak intensity between the left and right sciatic nerves. These results demonstrate the feasibility of CEUS to assess blood perfusion of the sciatic nerve in healthy dogs qualitatively and quantitatively. This result from healthy dogs could serve as a reference for further studies that evaluate the sciatic nerve under pathological conditions.