Both pectin and pectinase are vitally imperative biomolecules in the biotechnological sector. These molecules are a feasible non-toxic contrivance of nature with extensive applicative perception. Understanding pectic substances and their structure, unique depolymerization, and biochemical properties such as a catalytic mechanism and the strong interrelationship among these molecules could immensely enhance their applicability in industries. For instance, gaining knowledge with respect to the versatile molecular heterogeneity of the compounds could be considered as the center of concern to resolve the industrial issues from multiple aspects. In the present review, an effort has been made to orchestrate the fundamental information related to structure, depolymerization characteristics, and classification of pectin as well as the types and biochemical properties of pectinase. Furthermore, various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article.
Malaria is one of the most prevalent vector borne infectious disease and a serious global health problem in the world. Treatment for malaria is commonly inadequate due to the lack of quality assured limited number of effective drugs, underline how important it is to discover new antimalarial plants from number of natural sources. In the present study, the efficacy of antimalarial activity was studied by taking six various (n-hexane, chloroform, petroleum ether, ethanol, methanol and aqueous) organic leaf extracts of (Clarke) Pamp. against malarial parasite. Promising antiplasmodial activity was found in all tested extracts; however, maximum 50% inhibitory concentration (IC) values were noticed after 32 h of incubation, which is 5.76 ± 0.82, 7.09 ± 1.09, 9.88 ± 1.13, 10.24 ± 1.52, 11.37 ± 1.77 and 50.15 ± 6.16 µg/ml in methanol, chloroform, n-hexane, petroleum ether, ethanol and aqueous extracts, respectively. In conclusion, leaf extract possesses antiplasmodial activity which may be used as a potent plant-based antimalarial drug in the future by investigating the hidden phytochemical/(s).
The outstretched application of biosensors in diverse domains has become the reason for its attraction to scientific communities. Being an analytical device, its functionality includes both quantitative and qualitative detection...
The present study was focused on the potentiality of agro-based residues for the production of pectinase to meet the growing market demand by improving the yield with low cost of production. Among the agro-based residues used for the production of pectinase, apple pomace was able to produce the maximum of 1366.30 ± 36.71 U/ml using Aspergillus parvisclerotigenus KX928754 in liquid static surface fermentation, followed by sugarcane bagasse (973.12 ± 22.43 U/ml) and used tea (686.7 ± 45.06 U/ml). The process parameters optimization using a single variable at a time affirmed that pH 7.0, incubation period of 168 h, 30°C temperature, sucrose 2% as carbon source and peptone 3% as nitrogen source was found to be optimum for better production. The crude filtrate was purified by precipitation, dialyzed, eluted on Sephadex G-100 column followed by lyophilization and stored at −20°C. A. parvisclerotigenus KX928754 pectinase was purified to 2.10-fold, 2.91% of yield rate and having a specific activity of 1081.66 U/mg. Moreover, the electrophoretic analysis through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed 37.4 kDa of protein from the purified pectinase. Thus, the use of apple pomace as a substrate for scaling up pectinase with efficient recovery could reduce the price of the enzyme and increase its avenue for different industrial exploitation.
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